| IED ID | IndEnz0002012346 |
| Enzyme Type ID | protease012346 |
| Protein Name |
Cysteine protease YopT EC 3.4.22.- |
| Gene Name | yopT YPCD1.20 y5059 y0065 YP_pCD67 |
| Organism | Yersinia pestis |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Yersinia Yersinia pseudotuberculosis complex Yersinia pestis |
| Enzyme Sequence | MNSIHGHYHIQLSNYSAGENLQSATLTEGVIGAHRVKVETALSHSNLQKKLSATIKHNQSGRSMLDRKLTSDGKANQRSSFTFSMIMYRMIHFVLSTRVPAVRESVANYGGNINFKFAQTKGAFLHKIIKHSDTASGVCEALCAHWIRSHAQGQSLFDQLYVGGRKGKFQIDTLYSIKQLQIDGCKADVDQDEVTLDWFKKNGISERMIERHCLLRPVDVTGTTESEGLDQLLNAILDTHGIGYGYKKIHLSGQMSAHAIAAYVNEKSGVTFFDPNFGEFHFSDKEKFRKWFTNSFWGNSMYHYPLGVGQRFRVLTFDSKEV |
| Enzyme Length | 322 |
| Uniprot Accession Number | O68703 |
| Absorption | |
| Active Site | ACT_SITE 139; ACT_SITE 258; ACT_SITE 274 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease, which is translocated into infected cells and plays a central role in pathogenesis by cleaving the C-terminus end of the human small GTPase RhoA/ARHA, a regulator of cytoskeleton. Once cleaved, ARHA loses its lipid modification, and is released from the cell membrane, leading to the subsequent disruption of actin cytoskeleton of the host cell. {ECO:0000269|PubMed:12062101}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Mutagenesis (7) |
| Keywords | Hydrolase;Plasmid;Protease;Reference proteome;Secreted;Thiol protease;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Note=In infected cells, it is cytoplasmic. Translocated into the host cell by the type III secretion apparatus with the help of the SycT chaperone. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pCD1 |
| Mass | 36,308 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |