| IED ID | IndEnz0002012385 |
| Enzyme Type ID | protease012385 |
| Protein Name |
Chloroplast processing peptidase EC 3.4.21.89 Signal peptidase I-3 |
| Gene Name | PLSP1 At3g24590 MOB24.17 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MMVMISLHFSTPPLAFLKSDSNSRFLKNPNPNFIQFTPKSQLLFPQRLNFNTGTNLNRRTLSCYGIKDSSETTKSAPSLDSGDGGGGDGGDDDKGEVEEKNRLFPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKVSYYFRKPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNEKFILEPPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRVSGTVLEGGCAVDKQ |
| Enzyme Length | 291 |
| Uniprot Accession Number | Q8H0W1 |
| Absorption | |
| Active Site | ACT_SITE 142; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.; EC=3.4.21.89; |
| DNA Binding | |
| EC Number | 3.4.21.89 |
| Enzyme Function | FUNCTION: Involved in the maturation of the plastid protein translocation channel. Required for the biogenesis of plastid internal membranes. May also function as a thylakoidal processing peptidase. {ECO:0000269|PubMed:16275749}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous initiation (1); Region (1); Sequence conflict (1); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Membrane;Plastid;Protease;Reference proteome;Thylakoid;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast envelope {ECO:0000269|PubMed:16275749}. Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:16275749}. Note=May be located at the envelope membranes in premature chloroplast and at the thylakoidal membrane in mature chloroplasts. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15028209; 15322131; 16895613; 18431481; 18650403; 19223000; 20097790; 21139083; 22087276; 23727204; 23802992; 25182596; 26446787; 27247031; 32169961; |
| Motif | |
| Gene Encoded By | |
| Mass | 32,554 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.89; |