| IED ID | IndEnz0002012386 |
| Enzyme Type ID | protease012386 |
| Protein Name |
Plasmepsin IX PfPMIX EC 3.4.23.- Plasmepsin 9 |
| Gene Name | PMIX PF3D7_1430200 |
| Organism | Plasmodium falciparum (isolate 3D7) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Aconoidasida Haemosporida (haemosporidians) Plasmodiidae Plasmodium Plasmodium (Laverania) Plasmodium falciparum Plasmodium falciparum (isolate 3D7) |
| Enzyme Sequence | MFFINFKKIKKKQFPIYLTQHRIITVFLIFIYFINLKDCFHINNSRILSDVDKHRGLYYNIPKCNVCHKCSICTHENGEAQNVIPMVAIPSKRKHIQDINKEREENKYPLHIFEEKDIYNNKDNVVKKEDIYKLRKKKKQKKNCLNFLEKDTMFLSPSHDKETFHINHMNKIKDEKYKQEYEEEKEIYDNTNTSQEKNETNNEQNLNINLINNDKVTLPLQQLEDSQYVGYIQIGTPPQTIRPIFDTGSTNIWIVSTKCKDETCLKVHRYNHKLSSSFKYYEPHTNLDIMFGTGIIQGVIGVETFKIGPFEIKNQSFGLVKREKASDNKSNVFERINFEGIVGLAFPEMLSTGKSTLYENLMSSYKLQHNEFSIYIGKDSKYSALIFGGVDKNFFEGDIYMFPVVKEYYWEIHFDGLYIDHQKFCCGVNSIVYDLKKKDQENNKLFFTRKYFRKNKFKTHLRKYLLKKIKHQKKQKHSNHKKKKLNKKKNYLIFDSGTSFNSVPKDEIEYFFRVVPSKKCDDSNIDQVVSSYPNLTYVINKMPFTLTPSQYLVRKNDMCKPAFMEIEVSSEYGHAYILGNATFMRYYYTVYRRGNNNNSSYVGIAKAVHTEENEKYLSSLHNKINNL |
| Enzyme Length | 627 |
| Uniprot Accession Number | Q8ILG2 |
| Absorption | |
| Active Site | ACT_SITE 246; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 495; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by small molecule 49c (PubMed:29074775). Inhibited by small molecule WM382 (PubMed:32109369). {ECO:0000269|PubMed:29074775, ECO:0000269|PubMed:32109369}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: During the asexual blood stage, initiates the proteolytic maturation of several rhoptry proteins and thus, is required for merozoite invasion of host erythrocytes and probably the subsequent development of the ring-stage (PubMed:29074775, PubMed:32109369). Cleaves rhoptry associated protein 1 RAP1 and apical sushi protein ASP during schizont maturation (PubMed:29074775, PubMed:32109369). Also cleaves rhoptry protein RON3 (PubMed:32109369). {ECO:0000269|PubMed:29074775, ECO:0000269|PubMed:32109369}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Mutagenesis (1); Propeptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Aspartyl protease;Cytoplasmic vesicle;Hydrolase;Membrane;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type II membrane protein {ECO:0000305}. Cytoplasmic vesicle, secretory vesicle, rhoptry {ECO:0000269|PubMed:29074775}. Note=In schizonts, localizes to the bulb of rhoptries. {ECO:0000269|PubMed:29074775}. |
| Modified Residue | |
| Post Translational Modification | PTM: Autocleaved into a p55 mature form. {ECO:0000269|PubMed:32109369}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 74,183 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |