| IED ID | IndEnz0002012388 |
| Enzyme Type ID | protease012388 |
| Protein Name |
Heparan sulfate 2-O-sulfotransferase pipe EC 2.8.2.- |
| Gene Name | pip CG9614 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MSLNAERSYKMKLRDVENAFKYRRIPYPKRSVELIALLAISCTFFLFMHTNKLNSRLKEMEVKLQPSEFSALGLTGNHISGHDAGKHDDINTLHGTYQYLKSTGQKVGYNVHDRRSSEEQLRTPTAHGHHHDHHSHHHHMHQQEKIDGHKHKASHDKQLAVPDNKHKEDEVHYEDDEDEVEENDDDLANDVGTTDSEGFNFKADLLNNTKYAEVDFVFFNRVPKVGSQSLMELMARLGKINGFTHARNKGSAHETIVMNKQRQNDLIADLLTRPKPHIYSQHIAYINFTRFHLPKPIYINLIRDPIDRIISWHYYIRAPWYYRDMQAKLGENAIPMPSEEFMNLDLDTCVRNHDPHCTFTQMQIKNPVGDHRRQTLFFCGMNQKLCMPFNSEAAMQKAKRTVETEYAVVGTWEDTNITLSVLEAYIPRYFRNAKVAYYLGKDRLSRVNRNNVTRIVSDETRLILRKNLTNEIEFYEFCKQRLYLQYAALSHGKRFGEDDYLLVPEQQNEYNEDY |
| Enzyme Length | 514 |
| Uniprot Accession Number | Q86BJ3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.8.2.- |
| Enzyme Function | FUNCTION: Sulfotransferase involved in dorsoventral axis patterning in early embryos. Required for the specific ventral activation of a series of proteases acting in the perivitelline space between the embryonic membrane and the eggshell. Probably acts by mediating the sulfation of some glycoprotein or glycosaminoglycan stably deposited in the egg, whose ventrally localized modification leads to spatially restricted activation of the protease cascade. {ECO:0000269|PubMed:9568717, ECO:0000269|PubMed:9827800}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (21); Chain (1); Compositional bias (3); Erroneous initiation (2); Erroneous translation (2); Glycosylation (5); Region (1); Sequence caution (1); Sequence conflict (1); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Developmental protein;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Down-regulated by gurken (grk) and EGF receptor signaling, preventing its expression in dorsal follicle cells. {ECO:0000269|PubMed:12050143, ECO:0000269|PubMed:9827800}. |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:11076773}; Single-pass type II membrane protein {ECO:0000269|PubMed:11076773}. Note=Wbl is required for its processing into the Golgi. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,260 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |