| IED ID | IndEnz0002012436 |
| Enzyme Type ID | protease012436 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 12 EC 3.4.19.12 Deubiquitinating enzyme 12 Ubiquitin thioesterase 12 Ubiquitin-specific-processing protease 12 |
| Gene Name | usp12 |
| Organism | Salmo salar (Atlantic salmon) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Euteleosteomorpha Protacanthopterygii Salmoniformes (salmons and trouts) Salmonidae (salmonids) Salmoninae (trouts salmons & chars) Salmo Salmo salar (Atlantic salmon) |
| Enzyme Sequence | MEILMTVSKFASFCTMGANASALEKEIGSEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKILAYRSQPRRKENLLTCLADLFHSIANQKRKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADLLQEERKQDKTNGRLANGSLDSQNHNSNAPPPSTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMRVKKLPMILALHLKRFKYMEQLQRYTKLSYRVVFPLELRLFNTSGDATNPERLYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSEISKNSESGYILFYQSRD |
| Enzyme Length | 372 |
| Uniprot Accession Number | C0HB46 |
| Absorption | |
| Active Site | ACT_SITE 48; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 319; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity. {ECO:0000250|UniProtKB:O75317}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Metal binding (4); Region (1); Sequence conflict (1) |
| Keywords | Hydrolase;Metal-binding;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,117 |
| Kinetics | |
| Metal Binding | METAL 188; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75317; METAL 191; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75317; METAL 235; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75317; METAL 238; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75317 |
| Rhea ID | |
| Cross Reference Brenda |