| IED ID | IndEnz0002012535 |
| Enzyme Type ID | protease012535 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase isozyme L5 UCH-L5 EC 3.4.19.12 Ubiquitin thioesterase L5 |
| Gene Name | uch2 uchl5 DDB_G0285527 |
| Organism | Dictyostelium discoideum (Slime mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold) |
| Enzyme Sequence | MSENEGWCTIESDPGVFTELITKIGVKDIQVEELYTLDSSEYDRLKPVLGLIFLFKWEKEEENRTISDNENIFFANQVIQNACATQAILSVLLNSEGIELGEELSNFKSFVGDFPPMMKGEAIGNSELIKETHNSFTVQDPFIFSKKKNRKPSDAFHFISFIPFQGKVYELDGLKKGPYCLGDCTPDNWLEIATPFIQKRMEKYSQGEIRFNLMAVIKNRQTTLQEKILTLEKKKNDLEIKLSELNSGSGGDNKEESGGATPTTKEDLNFMINVVNNDIEEANNEILMEQEKFRNWKDENIRRKHNFTPLILNLIKGLAEKDNLQPLIQKAKDQISQKQQQHK |
| Enzyme Length | 343 |
| Uniprot Accession Number | Q54N38 |
| Absorption | |
| Active Site | ACT_SITE 83; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 157; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Region (1); Site (1) |
| Keywords | Cytoplasm;Hydrolase;Nucleus;Protease;Proteasome;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,325 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |