Detail Information for IndEnz0002012545
IED ID IndEnz0002012545
Enzyme Type ID protease012545
Protein Name Vacuolar-processing enzyme
VPE
EC 3.4.22.-
Legumain-like proteinase
LLP
Gene Name
Organism Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade indigoferoid/millettioid clade Phaseoleae Phaseolus Phaseolus vulgaris (Kidney bean) (French bean)
Enzyme Sequence MATTTATTSLLALLLLFLVALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPNGDEVYKGVPKDYTGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPGEDPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLVKERTISGGLYYGSHVMQYGDVGLSKDILFHYLGTDPANENLTFVDENSLWSSSKAVNQRDADLVHFWDKFRKAPEGSPKKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKAPELLNAVRPAGSALVDDWDCLKTMVRTFETHCGSLSQYGMKHMRSFANMCNVGIKKEQMREASAQACVTIPANPWSSLQRGFSA
Enzyme Length 484
Uniprot Accession Number O24325
Absorption
Active Site ACT_SITE 167; /evidence=ECO:0000255; ACT_SITE 209; /evidence=ECO:0000255
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.22.-
Enzyme Function FUNCTION: Asparagine-specific endopeptidase. Probably involved in the degradation of phaseolin during and after germination. {ECO:0000269|PubMed:9874222}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Propeptide (2); Signal peptide (1)
Keywords Direct protein sequencing;Hydrolase;Protease;Signal;Thiol protease;Zymogen
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..24; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 53,389
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.22.34;