| IED ID | IndEnz0002012556 |
| Enzyme Type ID | protease012556 |
| Protein Name |
Cysteine protease XCP1 EC 3.4.22.- Xylem cysteine peptidase 1 AtXCP1 |
| Gene Name | XCP1 At4g35350 F23E12.90 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAFSAPSLSKFSLLVAISASALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTFNSGCNGGLMDYAFQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMASYPTKTK |
| Enzyme Length | 355 |
| Uniprot Accession Number | O65493 |
| Absorption | |
| Active Site | ACT_SITE 161; /evidence=ECO:0000255|PROSITE-ProRule:PRU10088; ACT_SITE 297; /evidence=ECO:0000255|PROSITE-ProRule:PRU10089; ACT_SITE 317; /evidence=ECO:0000255|PROSITE-ProRule:PRU10090 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease involved in xylem tracheary element (TE) autolysis during xylogenesis in roots. Participates in micro autolysis within the intact central vacuole before mega autolysis is initiated by tonoplast implosion. {ECO:0000269|PubMed:18573193}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Glycosylation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Alternative splicing;Autocatalytic cleavage;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Signal;Thiol protease;Vacuole;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:11788755}. Cell membrane {ECO:0000269|PubMed:11788755}. Note=Predominantly vacuolar. May be associated to plasma membrane. |
| Modified Residue | |
| Post Translational Modification | PTM: Autocleaves. |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14585825; 14617066; 14697270; 15923329; 16061508; 16081527; 16214898; 16299169; 17217462; 17329564; 17565617; 17890373; 21284754; 22371507; 28921082; 31313414; |
| Motif | |
| Gene Encoded By | |
| Mass | 39,618 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |