| IED ID | IndEnz0002012580 |
| Enzyme Type ID | protease012580 |
| Protein Name |
Snake venom serine protease AHP-Ka SVSP EC 3.4.21.- Fragment |
| Gene Name | |
| Organism | Gloydius halys (Chinese water mocassin) (Agkistrodon halys) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Gloydius Gloydius halys (Chinese water mocassin) (Agkistrodon halys) |
| Enzyme Sequence | VIGGDECNINEHRFL |
| Enzyme Length | 15 |
| Uniprot Accession Number | P0DJG5 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by PMSF, Fe(3+), Fe(2+), Cu(2+), Cd(2+), Mn(2+), and Al(3+). Not inhibited by Ca(2+) and Mg(2+) and EDTA. {ECO:0000269|PubMed:22162083}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Snake venom serine protease that hydrolyzes specific chromogenic substrate S-2302 suggesting that AHP-Ka is a plasma kallikrein. Has esterase activity on alpha-N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) and amidolytic activity. {ECO:0000269|PubMed:22162083}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 30-40 degrees Celsius. {ECO:0000269|PubMed:22162083}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-8. {ECO:0000269|PubMed:22162083}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (1); Domain (1); Non-terminal residue (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. Cleavage of N-glycans reduces the catalytic enzymatic efficiency. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,716 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |