| IED ID | IndEnz0002012591 |
| Enzyme Type ID | protease012591 |
| Protein Name |
Snake venom metalloproteinase adamalysin-2 SVMP EC 3.4.24.46 Adamalysin II Proteinase II |
| Gene Name | |
| Organism | Crotalus adamanteus (Eastern diamondback rattlesnake) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Crotalus Crotalus adamanteus (Eastern diamondback rattlesnake) |
| Enzyme Sequence | QQNLPQRYIELVVVADRRVFMKYNSDLNIIRTRVHEIVNIINGFYRSLNIDVSLVNLEIWSGQDPLTIQSSSSNTLNSEGLWREKVLLNKKKKDNAQLLTAIEFKCETLGKAYLNSMCNPRSSVGIVKDHSPINLLVAVTMAHELGHNLGMEHDGKDCLRGASLCIMRPGLTPGRSYEFSDDSMGYYQKFLNQYKPQCILNKP |
| Enzyme Length | 203 |
| Uniprot Accession Number | P34179 |
| Absorption | |
| Active Site | ACT_SITE 144; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of 1-Phe-|-Val-2, 5-His-|-Leu-6, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, and 16-Tyr-|-Leu-17 of insulin B chain.; EC=3.4.24.46; |
| DNA Binding | |
| EC Number | 3.4.24.46 |
| Enzyme Function | FUNCTION: Has no significant hemorrhagic activity, but inactivates serpins by limited proteolysis of their reactive-site loops. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (7); Chain (1); Disulfide bond (2); Domain (1); Helix (8); Metal binding (7); Turn (3) |
| Keywords | 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1IAG; 2AIG; 3AIG; 4AIG; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,076 |
| Kinetics | |
| Metal Binding | METAL 10; /note=Calcium; /evidence=ECO:0000269|PubMed:9521103; METAL 94; /note=Calcium; /evidence=ECO:0000269|PubMed:9521103; METAL 143; /note=Zinc; catalytic; METAL 147; /note=Zinc; catalytic; METAL 153; /note=Zinc; catalytic; METAL 198; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000269|PubMed:9521103; METAL 201; /note=Calcium; /evidence=ECO:0000269|PubMed:9521103 |
| Rhea ID | |
| Cross Reference Brenda |