| IED ID | IndEnz0002012593 |
| Enzyme Type ID | protease012593 |
| Protein Name |
Snake venom metalloproteinase HT-2 SVMP EC 3.4.24.48 Hemorrhagic metalloproteinase HT-2 Hemorrhagic toxin II Ruberlysin |
| Gene Name | |
| Organism | Crotalus ruber ruber (Red diamond rattlesnake) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Crotalus Crotalus ruber (red diamond rattlesnake) Crotalus ruber ruber (Red diamond rattlesnake) |
| Enzyme Sequence | QNLPQSYIELVVVADHRMFMKYNSDLNTIRTRVHEIVNFINEFYRSLNIRVSLTDLEIWSDQDFITVQSSAKNTLHSFGEWRKSVLLNRKRHDNAQLLTAIVLDDYTLGLAYLNSMCHPRNSVGLIQDHSPINLLMGVTMAHELGHNLGMEHDGKDCLRGASLCIMRPGLTPGRSYEFSDASMRYYQKFLDQYKPQCILNKP |
| Enzyme Length | 202 |
| Uniprot Accession Number | P20897 |
| Absorption | |
| Active Site | ACT_SITE 143; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of 10-His-|-Leu-11, 14-Ala-|-Leu-15, 16-Tyr-|-Leu-17 and 23-Gly-|-Phe-24 bonds of the B chain of insulin, His-|-Pro, Pro-|-Phe, and Trp-|-Ser of angiotensin I, and Gly-|-Phe of Met enkephalin.; EC=3.4.24.48; |
| DNA Binding | |
| EC Number | 3.4.24.48 |
| Enzyme Function | FUNCTION: This protein is a zinc protease from snake venom that induces hemorrhage. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Domain (1); Metal binding (7); Modified residue (1) |
| Keywords | Calcium;Direct protein sequencing;Disulfide bond;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Protease;Pyrrolidone carboxylic acid;Secreted;Toxin;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | MOD_RES 1; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:2081731 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,321 |
| Kinetics | |
| Metal Binding | METAL 9; /note=Calcium; /evidence=ECO:0000250; METAL 93; /note=Calcium; /evidence=ECO:0000250; METAL 142; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 146; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 152; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 197; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 200; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |