| IED ID | IndEnz0002012618 |
| Enzyme Type ID | protease012618 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 36 EC 3.4.19.12 Deubiquitinating enzyme 36 Protein scrawny Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
| Gene Name | scny Usp36 CG5505 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MPVSMAVCETANVVNAALRESLGGNSSAGSSTDQAKSGEDTNGSLQNHIVANAKRILMAKIEYEEVPNYHESVLENLKSKYIVIKPGNPGAINGFSGKNNTGKLVGANGHDNNGARKQAEHPNNQSHHINHHNHQHPTSNPNELPKPKRVLYPRENIRIGWKQSERKWQVGTGMINVGNTCYLNSTLQALLHIPALANWLVSEQAHLADCNVAEPGSGCIICAMTKTLLATQSNQSAVRPFLIYSKLKQICKHMVVGRQEDAHEFLRFLVEAMERAYLMRFRNYKELDQLVKETTPLGQIFGGYLRSEVRCLSCNHVSITFQHFQDLLLDIRKADSLEDAFEGHFSRERLEDMGYKCEGCKKKVSATKQFSLERAPITLCIQLKRFSMIGNKLTKQISFKSRIDLSKYAARSQAAQAQPLTYRLVSMVTHLGASQHCGHYTAIGSTDTGSFYNFDDSYVRPIAMHSVCNTNAYIMFFELDLSQAASPAANRPNGVRLTNGHSTTPVPAATVSSPSPTRFIGPQLPAGGANGYTNGNAQKTAIQFKQQNQQSPQNGLQLGTGKFQDTAKPPLVGAHAKGEATSAPTANGNKSSSPSSNSSSNHKSINQQQYLPISSDDEDIEDEMKPRPTTAQLPSMPNMTENHTEPKAKSPVKIQVKTPVKTPLKSLVPYESASEEEEAPLPNPRKRPSGEDSSESDQESGQTNGHSKTNGSHTNGSASSSVHVNNSKQKTDAIDEIFKSLKKSADSDEDDDEEEPSIQLTNGWHPQKQSQSQSKAPPSPKTPPSPAVIKSKTGIWKVTRNDEVDAIEDDVDVVVVEGSPVKIPTPNKNHRNPFSSSKPSTDSPATPGAKRQKLLNGSALKSHQQPRVGNGYQSNATSNGSTINELLKQSYRGYGSPVLSWNGKPAELEKELLVDAREQRQRDIDDDEENEMDRGRQRKVKSGSAKGNNASNSTPGYNPFQEYEGQKRWNKNGGGGGFPRFYNQNYRQNFQQRNKFKFNRFGGPGSAKFQQQRALQRHLSAGGGFSRRQPSAQQQQQT |
| Enzyme Length | 1038 |
| Uniprot Accession Number | Q9VRP5 |
| Absorption | |
| Active Site | ACT_SITE 181; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01035, ECO:0000305|PubMed:19039105, ECO:0000305|PubMed:19837371"; ACT_SITE 439; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093, ECO:0000305|PubMed:19837371" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that deubiquitinates polyubiquitinated target proteins including imd (PubMed:19837371, PubMed:19039105). Required for preventing the constitutive activation of the imd/NF-kappa-B (Imd) signaling cascade under unchalleneged conditions (PubMed:19837371, PubMed:25027767). Deubiquitinates imd linked 'Lys-63' chains which leads its proteasomal degradation and consequently down-regulation of the Imd signaling cascade (PubMed:19837371). Removal of the activating 'Lys-63'-linked chains is likely to enable their replacement with 'Lys-48'-linked chains which act as 'tags' the for proteosomal degradation of imd (PubMed:19837371). Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells (PubMed:19039105). May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4me3) (PubMed:19039105). Controls selective autophagy activation by ubiquitinated proteins (PubMed:22622177). {ECO:0000269|PubMed:19039105, ECO:0000269|PubMed:19837371, ECO:0000269|PubMed:22622177, ECO:0000269|PubMed:25027767}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (5); Chain (1); Compositional bias (12); Domain (1); Modified residue (14); Mutagenesis (2); Region (6); Sequence conflict (4) |
| Keywords | Alternative splicing;Cytoplasm;Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:19039105}. Cytoplasm {ECO:0000269|PubMed:19837371}. |
| Modified Residue | MOD_RES 513; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 515; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 658; /note=Phosphothreonine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 662; /note=Phosphothreonine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 672; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 674; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 747; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 779; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 782; /note=Phosphothreonine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 785; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 819; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 825; /note=Phosphothreonine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 843; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 846; /note=Phosphothreonine; /evidence=ECO:0000269|PubMed:18327897 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10471706; 11102377; 12593803; 12695332; 16107480; 16157870; 18407920; 19026789; 19217892; 20220848; 20371351; 21074052; 21641546; 22253573; 22494833; 22724070; 22884528; 22912810; 22937016; 23071443; 23087835; 23124073; 23173095; 23275879; 23721820; 23899565; 24244183; 24362437; 24380076; 24576427; 24777180; 24949430; 25242144; 25294943; 25294944; 25628309; 26120032; 26431326; 26483546; 26507797; 26551273; 26979755; 27124581; 27507044; 27866148; 28084990; 28441530; 28674274; 29050293; 29146454; 30012668; 31068592; 31722958; 32573431; 32637412; 32656090; 32774336; 33561251; 33988679; 9584098; |
| Motif | |
| Gene Encoded By | |
| Mass | 114,088 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |