| IED ID | IndEnz0002012620 |
| Enzyme Type ID | protease012620 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 36 EC 3.4.19.12 Deubiquitinating enzyme 36 Protein scrawny Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
| Gene Name | Usp36 scny GM14719 |
| Organism | Drosophila sechellia (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila sechellia (Fruit fly) |
| Enzyme Sequence | MPVSMAVCETANVVNAALRESLGGNSSAGSSTDQAKSGEDTNGSLQNHIVANAKRILMAKIEYEEVPNYHESVLENLKSKYIVIKPGNPGAINGFSGKNNTGKLVGANGHDNNGARKQAEHPNNQSHHNHPHPTSNPNELPKPKRVLYPRENIRIGWKQSERKWQVGTGMINVGNTCYLNSTLQALLHIPALANWLVSEQAHLADCNVAEPGSGCIICAMAKTLLATQSNQSAVRPFLIYSKLKQICKHMVVGRQEDAHEFLRFLVEAMERAYLMRFRNYKELDQLVKETTPLGQIFGGYLRSEVRCLSCNHVSITFQHFQDLLLDIRKADSLEDAFEGHFSRERLEDMGYKCEGCKKKLKRFSMIGNKLTKQISFKPRIDLSKYAARSPAAQAQPLTYRLVSMVTHLGASQHCGHYTAIGSTDTGSFYNFDDSYVRPITMQNVCNTNAYIMFFELDLSQAASPPANRPNGVRLTNGHSTTPVPAATVSSPSPTRFIGPQLPPGGANGYTNGNAQKTAIQFKQQNQQNGLQLGTGKFQDTAKPPLVGAYAKGEATSAPTANGNKSSSPSSNSSSNHKSINQQQYLPISSDDEDIDDEMKPGPTTAQLPSMPNMTEDNTEPKAKSPVKIHLKTPVKTPLKSLVPYESASEEEEAPLPNPRQSPGGEDFSESDQESGQTNGHSKTNGSLTNGSASSSVHVNNSKQKTDAIDEIFKSLKKSADSEEDDDEEEPSIQLTNGWHPQKQSQSQSKAPPSPKTPPSPAVIKSKTGIWKVTRKDEVDAIDDDDDAVVVEGAPVKIPTPNKTHRNPFSSSKPSTDSPATPGAKRQKLLNGSALKSHQQPRVGNGYQSNVTSNGSTVNELLKQSYRGYGASVLSWNGKPAELEKEPFELVCAKRIAGHGSVEGSDIVEGSVAVDAAVTSSSNSNDVVVIAVALLVDAREQRQRDLDDDEENEMDRGRQRKVKSGSAKGNNASNSTPGYNPFQEYEGQKRWNKNGGGGGFSRFYNQNYRQNFQQRNKFKFNRFGGAGSAKFQQQRALQRHLSAGGGFSRRQPSAQQQQQT |
| Enzyme Length | 1059 |
| Uniprot Accession Number | B4HUI5 |
| Absorption | |
| Active Site | ACT_SITE 177; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 416; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Controls selective autophagy activation by ubiquitinated proteins. {ECO:0000250|UniProtKB:Q9VRP5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (10); Domain (1); Modified residue (13); Region (6) |
| Keywords | Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. |
| Modified Residue | MOD_RES 490; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 492; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 632; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 636; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 646; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 648; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 721; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 753; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 756; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 759; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 799; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 817; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 820; /note=Phosphothreonine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 115,591 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |