| IED ID | IndEnz0002012640 |
| Enzyme Type ID | protease012640 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 14 EC 3.4.19.12 Deubiquitinating enzyme 14 Ubiquitin thioesterase 14 Ubiquitin-specific-processing protease 14 |
| Gene Name | usp-14 tgt-1 C13B4.2 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MPIVNVKWQKEKYVVEVDTSAPPMVFKAQLFALTQVVPERQKVVIMGRTLGDDDWEGITIKENMTIMMMGSVGEIPKPPTVLEKKQANRDKQAEEISALYPCGLANLGNTCYFNSCVQMLKEVNELVLKPAEEMRIREHNDRLCHNLATLFNSLRDKDRALRSKGEPIKPFAAILTLSDSFPQFEKFKQQDANECLVSIMSNVTRIYGLSGWNIESLFRIQTETTMKCLESDEVSEKKVERNNQLTCYVNQDVRFLQTGIKAGFEEEMTRNSEELNRDAKWQKNTQISRLPKYLTVNINRFFYKESTKTNAKILKSVQFPMQLDTYDLCSQELKDKLVARRADIKLEEDAKLERELRKKVLDKEQGDKIFDDGVALPTAFEDDAGSNNSGFYDLKGIITHKGRSSQDGHYVAWMRSSEDGKWRLFDDEHVTVVDEEAILKTSGGGDWHSAYVLLYEARVIKQFPELPPAPVPTEVAADTAEPMEVSEKQ |
| Enzyme Length | 489 |
| Uniprot Accession Number | Q17361 |
| Absorption | |
| Active Site | ACT_SITE 111; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 409; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Region (1); Sequence conflict (1) |
| Keywords | Hydrolase;Protease;Proteasome;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 21085631; 21177967; 21367940; 22267497; 22560298; 23800452; 24884423; 25487147; 26009280; 27294288; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 55,878 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |