| IED ID | IndEnz0002012656 |
| Enzyme Type ID | protease012656 |
| Protein Name |
DNA-dependent metalloprotease WSS1 homolog EC 3.4.24.- DNA damage response protein WSS1 homolog |
| Gene Name | SPAC521.02 |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MIAILYLYYILTTSILLSVSFMLRINDDDHPNEKIGFISAIKGDFHDLSSDYLKRIAAMAFPIMKEHGFGVTSLDEVAYNAKFWGRNWNKGECIELVLRDASNRWLPFEFVMDVFLHELCHIWQGPHDRRFFSHLSTLRAALIALYAKGYKGPGKYMTWDSFVLANVVGNYNTVVFNGITLERSTMHGVETCGGSLQRKKKIRRKPTPSSTKKRKLTRTGQKLGTDMNIRLELLKSPAKPQAQSMRGREARIAAALLRVDNSNEYKPKDHNSSTTLENYFVVE |
| Enzyme Length | 283 |
| Uniprot Accession Number | Q9P7B5 |
| Absorption | |
| Active Site | ACT_SITE 118; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Metalloendopeptidase that acts selectively on DNA-binding proteins. DNA is needed to bring the protease and substrates together to enable proteolysis. Involved in the repair of toxic DNA-protein cross-links (DPCs) such as covalently trapped topoisomerase 1 (top1) adducts on DNA lesions or DPCs induced by reactive compounds such as formaldehyde. Involved in DNA damage response and processing of stalled or collapsed replication forks by removing the covalently trapped top1 from chromatin. DPC proteolysis enables the repair of the lesions via downstream DNA repair pathways. May be recruited to DPCs via the SUMOylation of substrate proteins at damaged DNA sites (By similarity). {ECO:0000250|UniProtKB:P38838}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Domain (1); Metal binding (3); Motif (1); Region (1) |
| Keywords | DNA damage;Hydrolase;Metal-binding;Metalloprotease;Nucleus;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20473289; 23697806; 30726745; 32034465; |
| Motif | MOTIF 256..263; /note=SUMO interaction motif (SIM); /evidence=ECO:0000250|UniProtKB:P38838 |
| Gene Encoded By | |
| Mass | 32,359 |
| Kinetics | |
| Metal Binding | METAL 117; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 121; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 127; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |