| IED ID | IndEnz0002012669 |
| Enzyme Type ID | protease012669 |
| Protein Name |
Zinc metalloproteinase/disintegrin Cleaved into: Snake venom metalloproteinase insularinase-A SVMP EC 3.4.24.- ; Disintegrin insularin |
| Gene Name | |
| Organism | Bothrops insularis (Golden lancehead) (Lachesis insularis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Bothrops Bothrops insularis (Golden lancehead) (Lachesis insularis) |
| Enzyme Sequence | MIQVLLVTICLAAFPYQGSSIILESGNVNDYEVVYARKVTELPKGAVQQKYEDAMQYEFKVNGEPVVLHLEKNKGLFSEDYSETHYSPDGRQIITYPPFEDHCYYHGRIENDADSTASISACNGLKGHFKLQGETYLIEPLKLPDSEAHAVYKYENVEKEDEAPKMCGVTETNWESYEPIEKASQSNLTPEQQKFSPRYIELAVVADHGMFTKYNSNLNTIRTRVHEMVNTLNGFFRSVNVDASLANLEVWSKKDLIKVEKDSSKTLTSFGEWRERDLLPRISHDHAQLLTTIVFDQQTIGLAYTAGMCDPRQSVAVVMDHSKKNLRVAVTMAHELGHNLGMDHDDTCTCGAKSCIMASTISKGLSFEFSKCSQNQYQTYLTDHNPQCILNKPLTTVSGNELLEAGEECDCGAPENPCCDAATCKLRPRAQCAEGLCCDQCRFKGAGKICRRARGDNPDDRCTGQSADCPRNRFHA |
| Enzyme Length | 476 |
| Uniprot Accession Number | Q5XUW8 |
| Absorption | |
| Active Site | ACT_SITE 335; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by EDTA, and 1,10-phenanthroline, but not by PMSF. {ECO:0000269|PubMed:16006246}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: [Snake venom metalloproteinase insularinase-A]: Non-hemorrhagic proteinase that activates prothrombin (F2) calcium-independently. Activates factor X (F10) and hydrolyzes the Aalpha-chain and more slowly the Bbeta-chain of fibrin and fibrinogen without affecting the gamma chain. It induces neither detachment nor apoptosis of human endothelial cells and is also not able to trigger an endothelial proinflammatory cell response. Nitric oxide and prostacyclin levels released by endothelial cells are significantly increased after treatment with insularinase A. {ECO:0000269|PubMed:16006246}.; FUNCTION: [Disintegrin insularin]: Inhibits platelet aggregation induced by ADP, thrombin, platelet-activating factor and collagen. Acts by inhibiting fibrinogen interaction with platelet receptors GPIIb/GPIIIa (ITGA2B/ITGB3) (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (2); Disulfide bond (7); Domain (2); Metal binding (7); Modified residue (1); Motif (1); Propeptide (2); Signal peptide (1) |
| Keywords | Blood coagulation cascade activating toxin;Calcium;Cell adhesion impairing toxin;Direct protein sequencing;Disulfide bond;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Platelet aggregation inhibiting toxin;Protease;Prothrombin activator;Pyrrolidone carboxylic acid;Secreted;Signal;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | MOD_RES 193; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000250 |
| Post Translational Modification | PTM: The N-terminus is blocked.; PTM: Not glycosylated. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 454..456; /note=Cell attachment site |
| Gene Encoded By | |
| Mass | 53,313 |
| Kinetics | |
| Metal Binding | METAL 201; /note=Calcium; /evidence=ECO:0000250; METAL 285; /note=Calcium; /evidence=ECO:0000250; METAL 334; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 338; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 344; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 388; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 391; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |