| IED ID | IndEnz0002012732 |
| Enzyme Type ID | protease012732 |
| Protein Name |
Serine protease 30 EC 3.4.21.- Distal intestinal serine protease Transmembrane serine protease 1 TMSP-1 Transmembrane serine protease 8 |
| Gene Name | Prss30 Disp Tmprss8 Tmsp-1 Tmsp1 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MESWARCIFLLLLQILTGGRGDILHSGAGKIVGGQDAPEGRWPWQVSLRTEKEGHICGGSLIHEVWVLTAAHCFRRPLNSSFYHVKVGGLTLSLTEPHSTLVAVRNIFVYPTYLWEDASSGDIALLRLDTPLQPSQFSPVCLPQAQAPLTPGTVCWVTGWGATHERELASVLQELAVPLLDSEDCERMYHIGETSLSGKRVIQSDMLCAGFVEGQKDSCQGDSGGPLVCAINSSWIQVGITSWGIGCARPNKPGVYTRVPDYVDWIQRTLAENHSDAYGCRSRASGAYPALLLVLLAFALPESL |
| Enzyme Length | 304 |
| Uniprot Accession Number | P83748 |
| Absorption | |
| Active Site | ACT_SITE 72; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P49863; ACT_SITE 122; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P49863; ACT_SITE 223; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P49863 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor. Partially inhibited by PMSF and DFP. {ECO:0000269|PubMed:14669991}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Selectively cleaves synthetic peptide substrates of trypsin. Activates the epithelial sodium channel ENaC. {ECO:0000269|PubMed:14669991}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9.0.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Domain (1); Glycosylation (3); Lipidation (1); Propeptide (2); Signal peptide (1) |
| Keywords | Cell membrane;Direct protein sequencing;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Ion transport;Lipoprotein;Membrane;Protease;Reference proteome;Serine protease;Signal;Sodium;Sodium transport;Transport;Zymogen |
| Interact With | |
| Induction | INDUCTION: By aldosterone. {ECO:0000269|PubMed:14669991}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-anchor {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 33,086 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |