| IED ID | IndEnz0002012800 |
| Enzyme Type ID | protease012800 |
| Protein Name |
Replicase polyprotein Cleaved into: Protein 1A CrPV-1A ; Protein 2A; Protein 2B; Protein 2C; Protein 3A; Protein 3B; 3C-like protease EC 3.4.22.- ; RNA-directed RNA polymerase 3D-POL EC 2.7.7.48 |
| Gene Name | ORF1 |
| Organism | Cricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Dicistroviridae Cripavirus Cricket paralysis virus (CrPV) Cricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV) |
| Enzyme Sequence | MSFQQTNNNATNNINSLEELAAQELIAAQFEGNLDGFFCTFYVQSKPQLLDLESECYCMDDFDCGCDRIKREEELRKLIFLTSDVYGYNFEEWKGLVWKFVQNYCPEHRYGSTFGNGLLIVSPRFFMDHLDWFQQWKLVSSNDECRAFLRKRTQLLMSGDVESNPGPVQSRPVYACDNDPRAIRLEKALQRRDEKISTLIKKLRQEIKNNRIYTQGFFDDLKGAKGEVGQLNGNLTRICDFLENSLPTLTAQIQTTVLTTTDKYVNLKEDLLKVAILLVLVRLLMVWKKYRAALIVIILFVMHFYGFDKQILDIVLDLKDKILQTTTQAGTETLEEVVYHPWFDTCGKLIFAVLAFFAIKKIPGKQDWDNYISRLDRIPKAIEGSKKIVDYCSEYFNLSVDEVKKVVLGKELKGTQGLYDEIHVWAKEIRHYLDLDERNKITLDTETAAKVEDLYKRGLKYSEEKIPDRDIARFITTMLFPAKSLYEQVLLSPVKGGGPKMRPITVWLTGESGIGKTQMIYPLCIDILREMGIVKPDAYKHQAYARQVETEYWDGYNGQKIVIYDDAFQLKDDKTKPNPEIFEVIRTCNTFPQHLHMAALQDKNMYSQAEVLLYTTNQFQVQLESITFPDAFYNRMKTHAYRVQIKQEKSIWVRNARGEEYNALDVTKLNKDEAIDLSVYEFQKMRFDDESATKWIDDGEPISYDEFARTICKAWKEEKEKTFHQLQWLEAYASRTVAQGGSETSEYYDVWDETYFSNLLSQGFMAGKSLIEMEAEFASDAETFNAYIEYKKNIPKETKWSKWMTILDEQISALSTKIRELKNKAYKFISEHPYLTALGFIGVMISAFAMYSFFERTLTDDTITSEVGSSGDNKTQKISKRVVEVGGSGDVKTTKPAKTAVEVGSSGDSKTMKNKITKVEVGSSGDSKTQKQRNTKVEVGKELEKEAETQGCSDPAAHALVLDVLQKNTYCLYYERMVKGEMKRYRLATATFLRGWVCMMPYHFIETLYARKVAPSTNIYFSQPNCDDVIVVPVSHFIAPNAERVELTTACTRIHYKDETPRDCVLVNLHRRMCHPHRDILKHFVKKSDQGNLRGVFQGTLATFHQSANELCRAYQWLQAIRPLDQEITIYHEDTDMFDYESESYTQRDCYEYNAPTQTGNCGSIVGLYNKRMERKLIGMHIPGNVSECHGYACPLTQEAIMDGLNRLEKLDPVNNITVQCCFEPPSDIKDTMSGETPEGKFCAIGKSNIKVGQAVKTTLLKSCIYGMLSKPITKPAHLTRTRLPNGEIVDPLMKGLKKCGVDTAVLDAEIVESAALDVKQVVLTQYNSMLDVNKYRRFLTYEEATQGTGDDDFMKGIARQTSPGYRYFQMPRKLPGKQDWMGSGEQYDFTSQRAQELRRDVEELIDNCAKGIIKDVVFVDTLKDERRPIEKVDAGKTRVFSAGPQHFVVAFRKYFLPFAAYLMNNRIDNEIAVGTNVYSTDWERIAKRLKKHGNKVIAGDFGNFDGSLVAQFFGQSCGKSFYPWFKTFNDVNTEDGKRNLMICIGLWTHIVHSVHSYGDNVYMWTHSQPSGNPFTVIINCLYNSMIMRIVWILLARKLAPEMQSMKKFRENVSMISYGDDNCLNISDRVVEWFNQITISEQMKEIKHEYTDEGKTGDMVKFPSLSEIHFLKKRFVFSHQLQRTVAPLQKDVIYEMLNWTRNTIDPNEILMMNINTAFREIVYHGKSEYQKLRSGIEDLAMKGILPQQPQILTFKAYLWDATMLADEVYDF |
| Enzyme Length | 1771 |
| Uniprot Accession Number | Q9IJX4 |
| Absorption | |
| Active Site | ACT_SITE 1003; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1063; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1162; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.4.22.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA. {ECO:0000269|PubMed:20400949}.; FUNCTION: RNA-directed RNA polymerase replicates genomic and antigenomic RNA. {ECO:0000255|PROSITE-ProRule:PRU00539, ECO:0000269|PubMed:20400949}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 510..517; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00551 |
| Features | Active site (3); Beta strand (3); Chain (9); Coiled coil (2); Domain (3); Helix (6); Nucleotide binding (1); Region (1); Site (7); Turn (2) |
| Keywords | 3D-structure;ATP-binding;Coiled coil;Helicase;Host-virus interaction;Hydrolase;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-binding;RNA-directed RNA polymerase;Reference proteome;Suppressor of RNA silencing;Thiol protease;Transferase;Viral RNA replication |
| Interact With | Q9VUQ5 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6C3R; |
| Mapped Pubmed ID | 30308158; |
| Motif | |
| Gene Encoded By | |
| Mass | 203,830 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |