| IED ID | IndEnz0002012824 |
| Enzyme Type ID | protease012824 |
| Protein Name |
Trypsin EC 3.4.21.4 |
| Gene Name | |
| Organism | Fusarium oxysporum (Fusarium vascular wilt) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Nectriaceae Fusarium Fusarium oxysporum species complex Fusarium oxysporum (Fusarium vascular wilt) |
| Enzyme Sequence | MVKFASVVALVAPLAAAAPQEIPNIVGGTSASAGDFPFIVSISRNGGPWCGGSLLNANTVLTAAHCVSGYAQSGFQIRAGSLSRTSGGITSSLSSVRVHPSYSGNNNDLAILKLSTSIPSGGNIGYARLAASGSDPVAGSSATVAGWGATSEGGSSTPVNLLKVTVPIVSRATCRAQYGTSAITNQMFCAGVSSGGKDSCQGDSGGPIVDSSNTLIGAVSWGNGCARPNYSGVYASVGALRSFIDTYA |
| Enzyme Length | 248 |
| Uniprot Accession Number | P35049 |
| Absorption | |
| Active Site | ACT_SITE 65; /note=Charge relay system; ACT_SITE 108; /note=Charge relay system; ACT_SITE 204; /note=Charge relay system |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (14); Chain (1); Disulfide bond (3); Domain (1); Helix (4); Propeptide (1); Signal peptide (1); Site (1); Turn (2) |
| Keywords | 3D-structure;Disulfide bond;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (17) |
| Cross Reference PDB | 1FN8; 1FY4; 1FY5; 1GDN; 1GDQ; 1GDU; 1PPZ; 1PQ5; 1PQ7; 1PQ8; 1PQA; 1TRY; 1XVM; 1XVO; 2G51; 2G52; 2VU8; |
| Mapped Pubmed ID | 16041079; 17327674; |
| Motif | |
| Gene Encoded By | |
| Mass | 24,576 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.4; |