| IED ID | IndEnz0002012834 |
| Enzyme Type ID | protease012834 |
| Protein Name |
Thrombin-like enzyme BJ-48 SVTLE BJ-48 EC 3.4.21.- Fibrinogen-clotting enzyme Snake venom serine protease SVSP Fragment |
| Gene Name | |
| Organism | Bothrops jararacussu (Jararacussu) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Bothrops Bothrops jararacussu (Jararacussu) |
| Enzyme Sequence | VVGGDCIPQVPFLAFLYSEYFC |
| Enzyme Length | 22 |
| Uniprot Accession Number | P0DJF0 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by aprotinin and dithiothreitol. {ECO:0000269|PubMed:17433397}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Thrombin-like serine protease which cleaves specifically the Aalpha chain of human fibrinogen (FGA) to release fibrinopeptide A. Also cleaves rapidly the Bbeta chain of human fibrinogen (FGB). Is selective for Arg over Lys at position 1 of the tripeptide substrates. {ECO:0000269|PubMed:17433397}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:17433397}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:17433397}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (2); Domain (1); Non-terminal residue (1); Sequence uncertainty (2) |
| Keywords | Blood coagulation cascade activating toxin;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Protease;Secreted;Serine protease;Sialic acid;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated; required for activity. Highly glycosylated with 42% of N-linked carbohydrates composed of Fuc(1):GalN(4):GlcN(5):Gal(1):Man(2) and a high content of sialic acid residues (8-12%). {ECO:0000269|PubMed:17433397}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,468 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.83 mM for BApNA {ECO:0000269|PubMed:17433397}; KM=0.4 mM for BAME {ECO:0000269|PubMed:17433397}; KM=0.6 mM for TAME {ECO:0000269|PubMed:17433397}; KM=0.3 mM for Nalpha-Tos-Gly-Pro-ME {ECO:0000269|PubMed:17433397}; KM=0.36 mM for Nalpha-Tos-Gly-Pro-Arg-pNA {ECO:0000269|PubMed:17433397}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |