| IED ID | IndEnz0002012871 |
| Enzyme Type ID | protease012871 |
| Protein Name |
26S proteasome regulatory subunit rpn11 Protein pad1 |
| Gene Name | rpn11 bfr2 pad1 sks1 SPAC31G5.13 |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MESLQRLLQGARMGTGMMGDQPLVDNSECVYISSLALLKMLRHGRHGTPMEVMGLMLGEFVDDFTVRVVDVFAMPQSGTGVSVEAVDPVFQKNMMDMLKQTGRPEMVVGWYHSHPGFGCWLSSVDINTQQSFEQLTPRAVAVVVDPIQSVKGKVVIDAFRLINPSTLMMGQEPRQTTSNLGHINKPSIQALIHGLGRHYYSLRINYKKTELEEIMLLNLHKQPWAHGLLLENFNSAAEKNHASIDKMKSLSEQYTERVQNEVTLSPEQLRIQYVGKQDPKKHLDAEVQKCIDNNISSMLACMLDSVAF |
| Enzyme Length | 308 |
| Uniprot Accession Number | P41878 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. {ECO:0000269|PubMed:9727008}.; FUNCTION: Transcription factor pap1 is controlled by the functional interaction between the positive regulator pad1 and negative regulator crm1. Both these proteins are also essential for cell viability and for the maintenance of chromosome structure. Pad1 is also responsible for resistance to staurosporine, and other drugs such as cycloheximide and caffeine. {ECO:0000269|PubMed:9727008}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Metal binding (3); Motif (1); Sequence conflict (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Protease;Proteasome;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10036242; 11084332; 12006658; 12553909; 14758541; 16096059; 18023413; 18257517; 19547744; 20133687; 20473289; 21182284; 23209828; 23697806; 25720772; 26412298; 29996109; 30726745; 8569688; 9374539; 9532803; 9822592; |
| Motif | MOTIF 112..125; /note=JAMM motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Gene Encoded By | |
| Mass | 34,572 |
| Kinetics | |
| Metal Binding | METAL 112; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 114; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 125; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Rhea ID | |
| Cross Reference Brenda |