| IED ID |
IndEnz0002012937 |
| Enzyme Type ID |
protease012937 |
| Protein Name |
Reversion-inducing cysteine-rich protein with Kazal motifs
|
| Gene Name |
reck |
| Organism |
Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Actinopterygii
Actinopteri
Neopterygii
Teleostei
Osteoglossocephalai
Clupeocephala
Otomorpha
Ostariophysi
Otophysi
Cypriniphysae
Cypriniformes (carps and others)
Cyprinoidei
Danionidae
Danioninae
Danio
Danio rerio (Zebrafish) (Brachydanio rerio)
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| Enzyme Sequence |
MSGCLQILTVLLCCRFWALVFSQDQSCCVHHAADIPRCRDACEQLASIRSESRLRHLLHRLPSYCPETLSELWICINNSLPGASRKSDGWVGLGCCELAISAECRRDCKQASSKNDISKVCKKDTENPLYSCITKNEMGSVCCSYAGRHTTCREYCQAIFRTDSSPTVSQISAVKEYCQSVSPPLILCVENYTRLHPTHRPIDSLHCCDRAEEAHCQLACKRILRTLSTEQEIMDGLISECGSQPLPQDPLWQCFLGSAHPPANTDPESPPIAKMDSAKLHCCFKANTSICRNMCVEISTSWGTQSWQEFDQHCEYNPVEMDLITCLADVREPCQLGCKELSYCTNFNNRPTELFRSCNVQSDQGALNDFKLWSNGSIRMPLMNIPVLDIRRCRPEMWKTVACALQIKPCYSRSRGSVICRSDCVEILRQCGDRRRFAEAQTPERICDLLSPTDDPERCIPLNRYLTASELESSVEEVIHPCNPNPCPSSHLCHVNRKGCHVGHDCLPYYCVPGCKLGEASEFLVPADARLQVPVHSAQPGCYEVCVCGQSGRLENCAEMPCFDTSKSCQIAGQRRSHGSSFRVDCNPCSCFAGDAVCSSRQCVRSDSSEEDRRLFTGLPCGCADHFVPVCAGNGRTYPSACVARCVGFTDSQFVFGSCRSFDPCSPNPCQRNQRCVPRRQVCLTDLSEFPCPQYECVSRPSSCDQKLLDPVCDTDNMEHANLCVLNLRGKTLAYSGHCQDACRRPREVCAHNGESYSTVCEAFSERVAVDYQGRCHAVGLESEFGSDSGCNAVPCPPLASDACQPVTPPGACCPVCAGMLRILWNKAQMNIFAKLNRDQPVSLHDILKILRLHVSVPQCDIFGYLSINSEIIILIAPVDQQPTPLQIEACSKEAEKIDSLINSGSPTLVSHVPLSAFLSSELQLSSVRSSSCVSISVCVLLLLCSLILTLTSDL |
| Enzyme Length |
955 |
| Uniprot Accession Number |
A0A0R4IKU3 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
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| Calcium Binding |
|
| catalytic Activity |
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| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Functions together with adgra2 to enable brain endothelial cells to selectively respond to Wnt7 signals (wnt7a or wnt7b) (PubMed:26051822, PubMed:26657775). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:26051822, PubMed:26657775). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7. Adgra2 is then required to deliver reck-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. Also acts as a serine protease inhibitor (By similarity). {ECO:0000250|UniProtKB:O95980, ECO:0000269|PubMed:26051822, ECO:0000269|PubMed:26657775}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
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| nucleotide Binding |
|
| Features |
Chain (1); Disulfide bond (5); Domain (3); Glycosylation (4); Lipidation (1); Mutagenesis (1); Propeptide (1); Region (1); Repeat (5); Signal peptide (1); Site (2) |
| Keywords |
Cell membrane;Disulfide bond;GPI-anchor;Glycoprotein;Lipoprotein;Membrane;Protease inhibitor;Reference proteome;Repeat;Serine protease inhibitor;Signal;Wnt signaling pathway |
| Interact With |
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| Induction |
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| Subcellular Location |
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26051822, ECO:0000269|PubMed:27979830}; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:A0A1D5PUP4}. Note=Colocalizes with adgra2 at the plasma membrane. {ECO:0000269|PubMed:26051822}. |
| Modified Residue |
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| Post Translational Modification |
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| Signal Peptide |
SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
22296847;
24098510;
29805750;
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| Motif |
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| Gene Encoded By |
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| Mass |
105,521 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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