| IED ID | IndEnz0002012938 |
| Enzyme Type ID | protease012938 |
| Protein Name |
Reversion-inducing cysteine-rich protein with Kazal motifs hRECK Suppressor of tumorigenicity 15 protein |
| Gene Name | RECK ST15 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MATVRASLRGALLLLLAVAGVAEVAGGLAPGSAGALCCNHSKDNQMCRDVCEQIFSSKSESRLKHLLQRAPDYCPETMVEIWNCMNSSLPGVFKKSDGWVGLGCCELAIALECRQACKQASSKNDISKVCRKEYENALFSCISRNEMGSVCCSYAGHHTNCREYCQAIFRTDSSPGPSQIKAVENYCASISPQLIHCVNNYTQSYPMRNPTDSLYCCDRAEDHACQNACKRILMSKKTEMEIVDGLIEGCKTQPLPQDPLWQCFLESSQSVHPGVTVHPPPSTGLDGAKLHCCSKANTSTCRELCTKLYSMSWGNTQSWQEFDRFCEYNPVEVSMLTCLADVREPCQLGCRNLTYCTNFNNRPTELFRSCNAQSDQGAMNDMKLWEKGSIKMPFINIPVLDIKKCQPEMWKAIACSLQIKPCHSKSRGSIICKSDCVEILKKCGDQNKFPEDHTAESICELLSPTDDLKNCIPLDTYLRPSTLGNIVEEVTHPCNPNPCPANELCEVNRKGCPSGDPCLPYFCVQGCKLGEASDFIVRQGTLIQVPSSAGEVGCYKICSCGQSGLLENCMEMHCIDLQKSCIVGGKRKSHGTSFSIDCNVCSCFAGNLVCSTRLCLSEHSSEDDRRTFTGLPCNCADQFVPVCGQNGRTYPSACIARCVGLQDHQFEFGSCMSKDPCNPNPCQKNQRCIPKPQVCLTTFDKFGCSQYECVPRQLACDQVQDPVCDTDHMEHNNLCTLYQRGKSLSYKGPCQPFCRATEPVCGHNGETYSSVCAAYSDRVAVDYYGDCQAVGVLSEHSSVAECASVKCPSLLAAGCKPIIPPGACCPLCAGMLRVLFDKEKLDTIAKVTNKKPITVLEILQKIRMHVSVPQCDVFGYFSIESEIVILIIPVDHYPKALQIEACNKEAEKIESLINSDSPTLASHVPLSALIISQVQVSSSVPSAGVRARPSCHSLLLPLSLGLALHLLWTYN |
| Enzyme Length | 971 |
| Uniprot Accession Number | O95980 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (PubMed:30026314). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:9789069, PubMed:18194466). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:9789069). {ECO:0000250|UniProtKB:Q9Z0J1, ECO:0000269|PubMed:18194466, ECO:0000269|PubMed:28289266, ECO:0000269|PubMed:30026314, ECO:0000269|PubMed:9789069}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (6); Domain (3); Glycosylation (5); Lipidation (1); Mutagenesis (1); Natural variant (1); Propeptide (1); Region (1); Repeat (5); Signal peptide (1) |
| Keywords | Cell membrane;Disulfide bond;GPI-anchor;Glycoprotein;Lipoprotein;Membrane;Protease inhibitor;Reference proteome;Repeat;Serine protease inhibitor;Signal;Tumor suppressor;Wnt signaling pathway |
| Interact With | P04626 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:30026314, ECO:0000269|PubMed:9789069}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:9789069}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000269|PubMed:9789069}. |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11072085; 12438739; 12738734; 12810630; 15328199; 16103099; 16377629; 16463672; 16791855; 16951151; 17033924; 17233834; 17262820; 17328864; 17329256; 17443689; 17583418; 17628776; 17714826; 18319621; 18425389; 18652766; 19022775; 19197139; 19208844; 19827606; 19913121; 20032402; 20127710; 20143471; 20154725; 20379614; 20395433; 20407016; 20480266; 20510080; 20579139; 20628086; 20711500; 20878580; 20973064; 21076843; 21254649; 21255571; 21432940; 21468925; 21516116; 21565829; 21613799; 21949998; 22025325; 22098593; 22139647; 22158033; 22178867; 22292753; 22321642; 22338226; 22397871; 22404079; 22419890; 22428065; 22438955; 22630347; 22761427; 22844865; 23176145; 23189844; 23226455; 23333633; 23383207; 23504349; 23679328; 23749490; 23820254; 23881612; 24078004; 24131772; 24227542; 24269686; 24335752; 24366472; 2443973; 24469470; 24510537; 24532189; 24647918; 24691523; 24931164; 25031745; 25278269; 25412941; 25465153; 25516656; 25537516; 25575057; 25826661; 25845681; 25877754; 25885317; 25987077; 26004948; 26026078; 26130413; 26141647; 26261088; 26284486; 26364844; 26431549; 26449734; 26459448; 26514267; 26921475; 26993249; 27058625; 27268601; 27272560; 27530410; 27696293; 28043014; 28340422; 28561419; 28740544; 29064588; 29108633; 29363862; 29599320; 29874120; 30733959; 30841433; 31074012; 31210300; 31270250; 31558368; 31764436; 31866421; 31933392; 32081769; 32138716; 32206004; 32255303; 32286475; 32522890; 32525042; 32661216; 32730638; 33447989; 33731436; 33781845; 34205376; 3422494; |
| Motif | |
| Gene Encoded By | |
| Mass | 106,457 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |