| IED ID | IndEnz0002012952 |
| Enzyme Type ID | protease012952 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase cyk-3 EC 3.4.19.12 CeUBP130 Cytokinesis defective protein 3 |
| Gene Name | cyk-3 uch-1 ZK328.1 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MGNTLTGRSNAIAPIISAEDAKTYISDEEYRRIRQAFQRFKNGCINYDEFCYHVLGGAQIPEEKRRLLFSFFSHGAETISFDNLLSSLVGLCRVEEVQSRFIEEYHEFASWGLSPPKLTIPLNDSYISFYEVMSYVTHLSVNEVIELEKVFATISDRAVCKLNEEKWKQALGGCFPDSYAERLFAVFDENRDGQIDFRELVCTLSALCRGPLPGRISQLARIWDVDCDKLLSDEELSNMYKDLNVPEEHQTVTKSSNGKSALVDFGIWAQENEKYVNEYYSMALQIGHICLGLRPESRKMELQIVNEFEKRASELPLSEWNIVASGWHAELRSFLEADKNPNPIDNSGIKGTREDSWTSKVACISAESARLKPDLIPSDYIRVPVPLWRAWLRWHGCALTVDSQFTRKYLDGEFFEDNKPALELYPLEILLLGHDRKKSQDGTENTPRSLTSWACAQVSRSMTVDELLALCKTELRLGDGDARLWQVVKENEEGNVLLDDGAQNLHQLYSSLGKTKKVNKMKLLLEVRERGTGVWPEELRASLSGKQITAASTLSSNAQLSDSSGRPGAVGLVNYGNFCYRNAGIQCLARVSPLTQYFLDEDNLDAIKRGNLRRGDAAETTIEYAKLLREMWAAKKKNIAPNDFNDAIRLSSDMFECSEQHDCQEFVAFLLDQLHTSMYESNKSLHPSPEESEGTDSNKLSDSSKKKEADKEEADEEKAERSWTEYEKQNESLVTQLFTGQLRSRLICRTCQSSSSVFEPFTSLSLPIGFEDVDLYQVIVVHRDGRIPRRYGFRLSRDSKVGNLREVVAVSSGISMSHLTIQCMSSKGTLMSRSPNHRSSNLRDELPLSSFPSGARLYALELPESTGEDQWRVAMHRKLQYNHEPYILGSTAGFIVSRFGLPLIVGLDEEVTGKKLYEDVMYQMHRFMEHSVNSSSSRAHDPCEDENSGYPFTLCLVDPNYEWCGQCPALRFCRGCPIRPDESKVFIPANCPIAVDWLPIALYLRYNHSQEQACEDDPSVAETWSRHFAPSSLEHCIEKFSCPETLDAAIQCDRCEKKTMRDKVMTIWKLPKYLIIHLKRFEFLREQGRMGKCKRTVNFPLKHFDPAPFVDKPDGNTYECIALANHYGQLSCGHFIAYAKSNEDKWLLLNDCSVREVSEEEVDKQGAYLLFYERKDVK |
| Enzyme Length | 1178 |
| Uniprot Accession Number | Q8WT44 |
| Absorption | |
| Active Site | ACT_SITE 579; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035; ACT_SITE 1134; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:11683918, ECO:0000269|PubMed:12006614}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Ubiquitin-protein hydrolase which cleaves ubiquitin from ubiquitinated proteins (PubMed:12006614, PubMed:11683918). Plays a role in embryo osmoregulation (PubMed:12006614). Probably by regulating osmosis, controls actin redistribution in the 1-cell embryos and thus actin-dependent processes such as cytokinesis and P-granules segregation (PubMed:12006614, PubMed:11683918). During the first embryonic mitotic division, involved in the formation of a functional microtubule organizing center provided by the male pronucleus (PubMed:11683918). {ECO:0000269|PubMed:11683918, ECO:0000269|PubMed:12006614}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Compositional bias (1); Domain (5); Metal binding (9); Mutagenesis (4); Region (1) |
| Keywords | Alternative splicing;Calcium;Cytoplasm;Cytoskeleton;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Repeat;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11683918}. Cytoplasm {ECO:0000269|PubMed:11683918}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000269|PubMed:11683918}. Note=At the 2-cell stage embryo, localizes to the microtubule organizing center during mitotic metaphase. Localizes to the cytoplasm in germ cells and sperm, and to the nucleus in oocytes. {ECO:0000269|PubMed:11683918}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 11099034; 12529635; 14551910; 15791247; 17164286; 18182484; 18692475; 19343510; 21085631; 21177967; 21529718; 22267497; 22286215; 22347378; 22560298; 23800452; 24884423; 25487147; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 134,123 |
| Kinetics | |
| Metal Binding | METAL 188; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 190; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 192; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 194; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 199; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 224; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 226; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 228; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 235; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448 |
| Rhea ID | |
| Cross Reference Brenda |