| IED ID | IndEnz0002013001 |
| Enzyme Type ID | protease013001 |
| Protein Name |
Trypsin-like protease try-5 EC 3.4.21.- Trypsin-like serine protease try-5 |
| Gene Name | try-5 K07B1.1 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MRPRIIVFLFQVLVVIKGTKLKYYNDELCGRQSTYTSFMLTDAAGNTGNPTHLAPWAVQIRVKARKGDFEVICGGTLITLKHVLTAAHCFQKHFGAKKEGGEENSMSGRYCESNQRFTDSEILTRTVVTVGAMCTRLEQKYGCVNEKQNGKTLKISRFAIGDFYKTHCEQGNDIVILELESTIDDVEGANYACLPFLPEVNIQSGANVTSFGWGSDPGKGFDNAAFPMIQVLTLATETLATCEENWGTSIPFDSFCTAEEEDKNVCSGDSGGGLTFHQSDSAREFIIAIVSYGSDCVQLIGGSEPRSQINTDVRKHQKFIVNFINQA |
| Enzyme Length | 327 |
| Uniprot Accession Number | O01887 |
| Absorption | |
| Active Site | ACT_SITE 88; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 173; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 270; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274 |
| Activity Regulation | ACTIVITY REGULATION: In the male gonad, probably maintained inactive by swm-1. {ECO:0000269|PubMed:22125495}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease which, in males, acts as a promoting signal during mating to activate sperm. {ECO:0000269|PubMed:22125495}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (1); Mutagenesis (3); Signal peptide (1) |
| Keywords | Cytoplasmic vesicle;Differentiation;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Spermatogenesis |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22125495, ECO:0000269|PubMed:30470702}. Cytoplasmic vesicle, secretory vesicle lumen {ECO:0000269|PubMed:22125495, ECO:0000269|PubMed:30470702}. Note=In males, partially colocalizes with swm-1 in vesicles near the apical membrane of cuboidal cells (PubMed:30470702, PubMed:22125495). During the spicule insertion stage of mating, secreted by the male vas deferens and transferred together with sperm into hermaphrodites where it spread into the uterus (PubMed:22125495). {ECO:0000269|PubMed:22125495, ECO:0000269|PubMed:30470702}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 11231151; 12445391; 21177967; 21367940; 22267497; 22560298; 23800452; 24324795; 24884423; 25487147; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,996 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |