| IED ID |
IndEnz0002013040 |
| Enzyme Type ID |
protease013040 |
| Protein Name |
Antitoxin YefM
|
| Gene Name |
yefM b2017 JW5835 |
| Organism |
Escherichia coli (strain K12) |
| Taxonomic Lineage |
cellular organisms
Bacteria
Proteobacteria
Gammaproteobacteria
Enterobacterales
Enterobacteriaceae
Escherichia
Escherichia coli
Escherichia coli (strain K12)
|
| Enzyme Sequence |
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE |
| Enzyme Length |
83 |
| Uniprot Accession Number |
P69346 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system. Antitoxin that counteracts the effect of the YoeB toxin. YefM binds to the promoter region of the yefM-yeoB operon to repress transcription, YeoB acts as a corepressor. {ECO:0000269|PubMed:14672926, ECO:0000269|PubMed:15009896, ECO:0000269|PubMed:17170003, ECO:0000269|PubMed:19028895}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (3); Chain (1); Helix (4); Mutagenesis (2) |
| Keywords |
3D-structure;DNA-binding;Reference proteome;Repressor;Toxin-antitoxin system;Transcription;Transcription regulation |
| Interact With |
|
| Induction |
INDUCTION: Represses its own promoter; more strongly repressed by the YefM(2)YoeB heterotrimer. Induced in persister cells. Ectopic expression of Salmonella or Shigella toxin VapC induces the yefM-yoeB operon and also induces Yoeb toxin activity in a Lon protease-dependent manner. {ECO:0000269|PubMed:16768798, ECO:0000269|PubMed:17170003, ECO:0000269|PubMed:19400780}. |
| Subcellular Location |
|
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
|
| Structure 3D |
X-ray crystallography (1) |
| Cross Reference PDB |
2A6Q;
|
| Mapped Pubmed ID |
24028219;
24561554;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
9,308 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|