| IED ID | IndEnz0002013042 |
| Enzyme Type ID | protease013042 |
| Protein Name |
Cysteine protease YopT EC 3.4.22.- |
| Gene Name | yopT pYV0041 |
| Organism | Yersinia pseudotuberculosis serotype I (strain IP32953) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Yersinia Yersinia pseudotuberculosis complex Yersinia pseudotuberculosis Yersinia pseudotuberculosis serotype I (strain IP32953) |
| Enzyme Sequence | MNSIHGHYHIQLSNYSAGENLQSATLTEGVIGAHRVKVETALSHSNLQKKLSATIKHNQSGRSMLDRKLTSDGKANQRSSFTFSMIMYRMIHFVLSTRVPAVRESVANYGGNINFKFAQTKGAFLHKIIKHSDTASGVCEALCAHWIRSHAQGQSLFDQLYVGGRKGKFQIDTLYSIKQLQIDGCKADVDQDEVTLDWFKKNGISERMIERHCLLRPVDVTGTTESEGLDQLLNAILDTHGIGYGYKKIHLSGQMSAHAIAAYVNEKSGVTFFDPNFGEFHFSDKEKFRKWFTNSFWDNSMYHYPLGVGQRFRVLTFDSKEV |
| Enzyme Length | 322 |
| Uniprot Accession Number | Q93RN4 |
| Absorption | |
| Active Site | ACT_SITE 139; /evidence=ECO:0000250; ACT_SITE 258; /evidence=ECO:0000250; ACT_SITE 274; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease, which is translocated into infected cells and plays a central role in pathogenesis by cleaving the C-terminus end of the human small GTPase RhoA/ARHA, a regulator of cytoskeleton. Once cleaved, ARHA loses its lipid modification, and is released from the cell membrane, leading to the subsequent disruption of actin cytoskeleton of the host cell. {ECO:0000269|PubMed:12538863}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Mutagenesis (1); Sequence conflict (2) |
| Keywords | Hydrolase;Plasmid;Protease;Secreted;Thiol protease;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=In infected cells, it is cytoplasmic. Translocated into the host cell by the type III secretion apparatus with the help of the SycT chaperone (By similarity). {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pYV36; Plasmid pYV |
| Mass | 36,366 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |