| IED ID | IndEnz0002013046 |
| Enzyme Type ID | protease013046 |
| Protein Name |
Retrovirus-related Pol polyprotein from transposon RE1 Retro element 1 AtRE1 Includes: Protease RE1 EC 3.4.23.- ; Reverse transcriptase RE1 EC 2.7.7.49 ; Endonuclease RE1 |
| Gene Name | RE1 RF12 RF28 At1g58889 R18I; RE1 At1g59265 T4M14.18 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAAHAEELVLNNTSILNVNMSNVTKLTSTNYLMWSRQVHALFDGYELAGFLDGSTTMPPATIGTDAAPRVNPDYTRWKRQDKLIYSAVLGAISMSVQPAVSRATTAAQIWETLRKIYANPSYGHVTQLRTQLKQWTKGTKTIDDYMQGLVTRFDQLALLGKPMDHDEQVERVLENLPEEYKPVIDQIAAKDTPPTLTEIHERLLNHESKILAVSSATVIPITANAVSHRNTTTTNNNNNGNRNNRYDNRNNNNNSKPWQQSSTNFHPNNNQSKPYLGKCQICGVQGHSAKRCSQLQHFLSSVNSQQPPSPFTPWQPRANLALGSPYSSNNWLLDSGATHHITSDFNNLSLHQPYTGGDDVMVADGSTIPISHTGSTSLSTKSRPLNLHNILYVPNIHKNLISVYRLCNANGVSVEFFPASFQVKDLNTGVPLLQGKTKDELYEWPIASSQPVSLFASPSSKATHSSWHARLGHPAPSILNSVISNYSLSVLNPSHKFLSCSDCLINKSNKVPFSQSTINSTRPLEYIYSDVWSSPILSHDNYRYYVIFVDHFTRYTWLYPLKQKSQVKETFITFKNLLENRFQTRIGTFYSDNGGEFVALWEYFSQHGISHLTSPPHTPEHNGLSERKHRHIVETGLTLLSHASIPKTYWPYAFAVAVYLINRLPTPLLQLESPFQKLFGTSPNYDKLRVFGCACYPWLRPYNQHKLDDKSRQCVFLGYSLTQSAYLCLHLQTSRLYISRHVRFDENCFPFSNYLATLSPVQEQRRESSCVWSPHTTLPTRTPVLPAPSCSDPHHAATPPSSPSAPFRNSQVSSSNLDSSFSSSFPSSPEPTAPRQNGPQPTTQPTQTQTQTHSSQNTSQNNPTNESPSQLAQSLSTPAQSSSSSPSPTTSASSSSTSPTPPSILIHPPPPLAQIVNNNNQAPLNTHSMGTRAKAGIIKPNPKYSLAVSLAAESEPRTAIQALKDERWRNAMGSEINAQIGNHTWDLVPPPPSHVTIVGCRWIFTKKYNSDGSLNRYKARLVAKGYNQRPGLDYAETFSPVIKSTSIRIVLGVAVDRSWPIRQLDVNNAFLQGTLTDDVYMSQPPGFIDKDRPNYVCKLRKALYGLKQAPRAWYVELRNYLLTIGFVNSVSDTSLFVLQRGKSIVYMLVYVDDILITGNDPTLLHNTLDNLSQRFSVKDHEELHYFLGIEAKRVPTGLHLSQRRYILDLLARTNMITAKPVTTPMAPSPKLSLYSGTKLTDPTEYRGIVGSLQYLAFTRPDISYAVNRLSQFMHMPTEEHLQALKRILRYLAGTPNHGIFLKKGNTLSLHAYSDADWAGDKDDYVSTNGYIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEMQWICSLLTELGIRLTRPPVIYCDNVGATYLCANPVFHSRMKHIAIDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLSRTAFQNFASKIGVTRVPPS |
| Enzyme Length | 1466 |
| Uniprot Accession Number | Q94HW2 |
| Absorption | |
| Active Site | ACT_SITE 334; /note=For protease activity; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.49; |
| DNA Binding | |
| EC Number | 3.4.23.-; 2.7.7.49 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (3); Domain (2); Frameshift (1); Metal binding (2); Region (2); Sequence conflict (3); Zinc finger (1) |
| Keywords | Aspartyl protease;DNA integration;DNA recombination;Endonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Protease;Reference proteome;Transferase;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 163,905 |
| Kinetics | |
| Metal Binding | METAL 530; /note=Magnesium; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00457; METAL 592; /note=Magnesium; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00457 |
| Rhea ID | RHEA:22508 |
| Cross Reference Brenda |