| IED ID | IndEnz0002013132 |
| Enzyme Type ID | protease013132 |
| Protein Name |
Snake venom metalloproteinase jararafibrase-4 SVMP EC 3.4.24.- Jararafibrase IV Fragment |
| Gene Name | |
| Organism | Bothrops jararaca (Jararaca) (Bothrops jajaraca) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Bothrops Bothrops jararaca (Jararaca) (Bothrops jajaraca) |
| Enzyme Sequence | TPEHQRYIELFLVVDHGMFMKYNGNSDKIYYYIHQMVNIMKXAYXYL |
| Enzyme Length | 47 |
| Uniprot Accession Number | P0C6S6 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by 1,10-phenanthroline and EDTA. {ECO:0000269|PubMed:8087204}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: The metalloproteinase is a probable venom zinc protease that induces local hemorrhage in the skin of rats. Degrades type-IV collagen, gelatin, laminin and fibronectin. Has fibrinolytic activities. Has high hemagglutinating activity on red blood cells. Cleaves insulin B chain at 29-His-|-Leu-30, and 38-Ala-|-Leu-39 bonds (PubMed:8087204). {ECO:0000269|PubMed:12165326, ECO:0000269|PubMed:8087204}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Metal binding (1); Non-terminal residue (1); Sequence conflict (1) |
| Keywords | Calcium;Direct protein sequencing;Fibrinolytic toxin;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Toxin;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 5,801 |
| Kinetics | |
| Metal Binding | METAL 9; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |