| IED ID | IndEnz0002013136 |
| Enzyme Type ID | protease013136 |
| Protein Name |
Vacuolar-processing enzyme beta-isozyme 1 Beta-VPE 1 OsVPE1 EC 3.4.22.34 Asparaginyl endopeptidase VPE1 |
| Gene Name | VPE1 Glup3 Os04g0537900 OsJ_15605 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MAARCWVWGFVVALLAVAAAADGEEEEGKWEPLIRMPTEEGDDAEAAAPAPAPAAADYGGTRWAVLVAGSSGYGNYRHQADVCHAYQILQKGGVKEENIVVFMYDDIAHNILNPRPGTIINHPKGGDVYAGVPKDYTGHQVTTENFFAVLLGNKTAVTGGSGKVIDSKPEDHIFIYYSDHGGPGVLGMPNLPYLYAGDFIKVLQKKHASNSYSKMVIYVEACESGSIFEGLMPENLNIYVTTASNAVENSWGTYCPGEEPSPPPEYITCLGDMYSVAWMEDSETHNLKKETIEDQYELVKKRTSNANKLNEGSHVMEYGDKTFKDEKLFLYQGFNPANGNITNELIWPVPKATVNQRDADLLFMWKRYEQLNGVSEDKLRALREIEDTIAHRKHLDSSIDFIGKLVFGFENGPLALEAARSSGQPLVDNWDCLKKMVRIFESQCGSLTQYGMKYMRAFANICNNGVSEAKMMEASINACGRYNSARWSPMTEGGHSA |
| Enzyme Length | 497 |
| Uniprot Accession Number | Q84LM2 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.; EC=3.4.22.34; Evidence={ECO:0000269|PubMed:19154227}; |
| DNA Binding | |
| EC Number | 3.4.22.34 |
| Enzyme Function | FUNCTION: Asparagine-specific endopeptidase that may be involved in processing of proteins targeted to vacuoles. Cysteine protease required for post-translational proteolysis of seed storage proteins in the protein storage vacuole (PSV) of developing seeds, by processing of proglutelin precursor to mature glutelin subunits, thus contributing to the formation of protein crystalline structures in PSV (Ref.1, PubMed:19154227, PubMed:19933265). {ECO:0000269|PubMed:19154227, ECO:0000269|PubMed:19933265, ECO:0000269|Ref.1}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (2); Mutagenesis (2); Signal peptide (1); Site (1) |
| Keywords | Glycoprotein;Hydrolase;Protease;Reference proteome;Seed storage protein;Signal;Storage protein;Thiol protease;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Protein storage vacuole {ECO:0000269|PubMed:19154227}. |
| Modified Residue | |
| Post Translational Modification | PTM: Auto-catalytic activation. {ECO:0000269|PubMed:19154227}. |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 21972902; |
| Motif | |
| Gene Encoded By | |
| Mass | 54,886 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |