| IED ID | IndEnz0002013149 |
| Enzyme Type ID | protease013149 |
| Protein Name |
Snake venom metalloproteinase trimerelysin-2 SVMP EC 3.4.24.53 H2 metalloproteinase H2-proteinase Trimerelysin II |
| Gene Name | |
| Organism | Protobothrops flavoviridis (Habu) (Trimeresurus flavoviridis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Protobothrops Protobothrops flavoviridis (Habu) (Trimeresurus flavoviridis) |
| Enzyme Sequence | QRFPQRYIELAIVVDHGMYKKYNQNSDKIKVRVHQMVNHINEMYRPLNIAISLNRLQIWSKKDLITVKSASNVTLESFGNWRETVLLKQQNNDCAHLLTATNLNDNTIGLAYKKGMCNPKLSVGLVQDYSPNVFMVAVTMTHELGHNLGMEHDDKDKCKCEACIMSDVISDKPSKLFSDCSKNDYQTFLTKYNPQCILNAP |
| Enzyme Length | 201 |
| Uniprot Accession Number | P20165 |
| Absorption | |
| Active Site | ACT_SITE 143 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of 3-Asn-|-Gln-4, 10-His-|-Leu-11 and 14-Ala-|-Leu-15 in the insulin B chain, and the bond Z-Gly-Pro-|-Leu-Gly-Pro in a small molecule substrate of microbial collagenase.; EC=3.4.24.53; |
| DNA Binding | |
| EC Number | 3.4.24.53 |
| Enzyme Function | FUNCTION: Major venom non-hemorrhagic metalloproteinase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (6); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (1); Helix (10); Metal binding (3); Modified residue (1); Turn (2) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Pyrrolidone carboxylic acid;Secreted;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | MOD_RES 1; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:2777746 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1WNI; |
| Mapped Pubmed ID | 8586633; |
| Motif | |
| Gene Encoded By | |
| Mass | 23,014 |
| Kinetics | |
| Metal Binding | METAL 142; /note=Zinc; catalytic; METAL 146; /note=Zinc; catalytic; METAL 152; /note=Zinc; catalytic |
| Rhea ID | |
| Cross Reference Brenda |