| IED ID | IndEnz0002013185 |
| Enzyme Type ID | protease013185 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 47 EC 3.4.19.12 Deubiquitinating enzyme 47 Ubiquitin thioesterase 47 Ubiquitin-specific-processing protease 47 |
| Gene Name | Usp47 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MVPGEENQLVPKEDVFWRCRQNIFDEMKKKFLQIENAAEEPRVLCIIQDTTNSKTVSERITLNLPASTPVRKLFEDVANKVGYINGTFDLTRENGVTTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEPPQMLLEDSNNVDDSVHDRFIGPLPREGSVASTNDYVSQNYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMSFPEELDMSTFIDIEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLESSSGSEKISKPGLEKNSLMYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPTRNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPVKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEDVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQIFQSYKPGEVMVKVHVVDLKAETVAAPVTVRAYLNQTVTEFKQLISKATHLPADSMRIVLERCYNDLRLLSMPSKTLKAEGFFRSNKVFVESSETVDHQAAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSYSKRTAYQKAGGDSGNVDDDCERVKGPAGNLKSVDAILEESTEKLKSLSLQQQQQDGDNGDSSKSTETSDFENIESPLNERGSSTSVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGDMQYMYFKADPYTADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYTSNQEFETVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVCQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLDLSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAILTRRWRPAEMKLDPFQELVLESNSVDELREKLSEISGIPLEDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDRTEEVMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDVAQD |
| Enzyme Length | 1376 |
| Uniprot Accession Number | Q8BY87 |
| Absorption | |
| Active Site | ACT_SITE 197; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 503; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER) (By similarity). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level. {ECO:0000250, ECO:0000269|PubMed:19966869}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Compositional bias (5); Domain (1); Erroneous initiation (2); Frameshift (1); Modified residue (7); Region (4); Sequence conflict (8) |
| Keywords | Acetylation;Alternative splicing;Cytoplasm;DNA damage;DNA repair;Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | MOD_RES 122; /note="N6-acetyllysine"; /evidence="ECO:0000250|UniProtKB:Q96K76"; MOD_RES 832; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:Q96K76"; MOD_RES 911; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:15345747, ECO:0007744|PubMed:21183079"; MOD_RES 934; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 1014; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 1016; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:17242355, ECO:0007744|PubMed:21183079"; MOD_RES 1018; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:Q96K76" |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11217851; 12466851; 12520002; 12904583; 14610273; 16602821; 16615898; 17967808; 21267068; 21677750; 27626380; 33397955; |
| Motif | |
| Gene Encoded By | |
| Mass | 157,455 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |