| IED ID | IndEnz0002013194 |
| Enzyme Type ID | protease013194 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase EC 3.4.19.12 Ubiquitin thioesterase |
| Gene Name | Uch ubc-D CG4265 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MLTWTPLESNPEVLTKYIHKLGVSPAWSVTDVIGLEDDTLEWIPRPVKAFILLFPCSETYEKHRAEEHDRIKEVEEQHPEDLFYMRQFTHNACGTVALIHSVANNKEVDIDRGVLKDFLEKTASLSPEERGRALEKDEKFTADHEALAQEGQTNAANHEKVIHHFIALVNKEGTLYELDGRKSFPIKHGPTSEETFVKDAAKVCKEFMARDPNEVRFTVLALTAAQQ |
| Enzyme Length | 227 |
| Uniprot Accession Number | P35122 |
| Absorption | |
| Active Site | ACT_SITE 93; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10091; ACT_SITE 164; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10091 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. {ECO:0000269|PubMed:7683284}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Sequence conflict (6); Site (1) |
| Keywords | Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10527495; 10603300; 10893261; 12610523; 12730221; 14973288; 15630418; 15899868; 17189251; 18056427; 18433294; 18817875; 18931788; 20220848; 20371351; 20547833; 20617173; 21074052; 22494833; 22526625; 22937016; 23071443; 23275879; 23607345; 23658762; 23899565; 24658702; 25020150; 25312911; 25708988; 26120032; 26290570; 26460970; 26526100; 26551273; 26870755; 26917723; 27582081; 27794539; 29397366; 29535397; 29951814; 29983616; 30057672; 30545438; 30719278; 31143437; 31585883; 31722958; 32357532; 32653676; 32956978; 33230107; 33988679; 34244144; 8631829; 9545464; |
| Motif | |
| Gene Encoded By | |
| Mass | 25,850 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |