| IED ID | IndEnz0002013227 |
| Enzyme Type ID | protease013227 |
| Protein Name |
Serralysin EC 3.4.24.40 Extracellular metalloprotease |
| Gene Name | zapA |
| Organism | Proteus mirabilis |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Morganellaceae Proteus Proteus mirabilis |
| Enzyme Sequence | MGSFLLKKAVGLSNISDLLDKSGIFYNYSTKVLPSFDYDTAGKHIAREDSTWNGKYVIGQPAEVTYSFPKWEGKFNQFGNKNPYEFNELQKEHARKSLDAWSDIANIKFTEVAVGNVDGMKASDVKTDITFGNIYDPNGTFQAYATLPNTYAYGKDLSGQAWFSDYHYAGNTTPELGNYGRLTIIHEIGHTLGLMHPGDYNAGQNVPGYLKSDYAEDSRQYTVMSYWDEYETGAHFQGAYAGAPLLHDISAMQYLYGANTTTRTGDDVYGFNSNTGIDYYTATDSNDKLIFSVWDSGGNDTFDFSGFYQDQLIDLRAGNFSDVGGLQKNVSIAQNVTIENAIGGFGNDIIHGNDADNTLIGGEGDDIIYGHSGNNTIYGGRGQDTLHGGTGSNTFIYKEIADSLVTAADKIMDFKTGIDKIDLSTLIQDTFSSKILNFVDNFTGNAGEATLSYNEVTNASELAINAYGYNYNPDFKIDIVGFVNYETDFIV |
| Enzyme Length | 491 |
| Uniprot Accession Number | Q11137 |
| Absorption | |
| Active Site | ACT_SITE 187; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | ACTIVITY REGULATION: Ca(2+) increases protease activity. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'.; EC=3.4.24.40; |
| DNA Binding | |
| EC Number | 3.4.24.40 |
| Enzyme Function | FUNCTION: One of the virulence factors produced during swarmer cell differentiation of the bacteria, which seems to be associated with pathogenesis. The protease activity is limited to IgA1, IgA2, as well as IgG degradation. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous initiation (1); Metal binding (34); Propeptide (1); Repeat (3) |
| Keywords | Calcium;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Virulence;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 54,000 |
| Kinetics | |
| Metal Binding | METAL 186; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 190; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 196; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 226; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 263; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 265; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 295; /note=Calcium 1; /evidence=ECO:0000250; METAL 297; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 298; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 300; /note=Calcium 1; /evidence=ECO:0000250; METAL 300; /note=Calcium 2; /evidence=ECO:0000250; METAL 337; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 339; /note=Calcium 2; /evidence=ECO:0000250; METAL 344; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 346; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 348; /note=Calcium 3; /evidence=ECO:0000250; METAL 353; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 355; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 357; /note=Calcium 4; /evidence=ECO:0000250; METAL 361; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 362; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 364; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 366; /note=Calcium 3; /evidence=ECO:0000250; METAL 366; /note=Calcium 5; /evidence=ECO:0000250; METAL 370; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 373; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250; METAL 384; /note=Calcium 4; /evidence=ECO:0000250; METAL 384; /note=Calcium 6; /evidence=ECO:0000250; METAL 388; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 389; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 391; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 402; /note=Calcium 6; /evidence=ECO:0000250; METAL 409; /note=Calcium 6; /evidence=ECO:0000250; METAL 419; /note=Calcium 7; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |