| IED ID | IndEnz0002013229 |
| Enzyme Type ID | protease013229 |
| Protein Name |
Probable ubiquitin carboxyl-terminal hydrolase 7 EC 3.4.19.12 Deubiquitinating enzyme 7 Ubiquitin thioesterase 7 Ubiquitin-specific-processing protease 7 |
| Gene Name | ubp7 SPAC23G3.08c |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MPHSNTLIVPSTNPFDDPSSIKNGLNNLTSSELVNQMKKESLAASVKTPSTPPKECSHLKKGVKLSHLKGNARALRDPSKHLCFICTTEKIKREDYELTLCANCGYLFCCNHESDHSRKHFEKNKKHCVFVNIITLKCHCYSCDADIVIFDKKNLVVRDVQQFICSNLVSSLTKAPNVLKSNSSHFKKEKKSKHSSGKSSKKYKVISPGLKNLGATCFFNSTLQVLCACEALHDVISPFQYSHSSVIVRKLTKSPESSLLSAFIKFLETFYKSDGTISVYRPTTFFGEFRRLHPQFSESVQQDAHELLRLLLDDLISEEFRVLRFNLNSVSRSLQLSPCLTDDEQLSKSLTSFKQVNVTDASLSPNSHNTSDNEQNNEDYVSVSSLVGSETEDITYSKELSQSSDSSQHQHDSFLPANSSPLAASSTKSLPSSELLDSSSDKGQQVFKGQHEVAGTNSFEDPNSHFNVSNSSNHEEASPKKEVLKSPQFQRRSLDILRLGELSSDDMMLDKATMDEFSSSLVIKSIFTGRLTSVVMCQSCNEITNTPEPIQDLSIPIHYPSSRVSRRHRFHRALRSRFSRSPKKSSVKIVVDNANDDTDQAPTTNSSSLNENLLGGHASENDKSLKQSPFQKLTRRLSDLSVNSSGQISKQDFDNSNSIFSESSLSSPIIEEPKTLIDCLKNFTHVEELSGENMFACENCCNQPNEVGSPAKGGLTSDNDKYSFNNSVYRNAYKRMLLDDPLPPVFIIHLKRFFQEISHDGYANPKKISDFIEFEQELDLNEFVMPHLRASSSFRYRLFGVIVHSGTLNYGHYVAYVLSHKFLDLSAPSTNSKDFRSEAGIPERRWLYISDNIVRESSWDEVSKVEAYMLFYERV |
| Enzyme Length | 875 |
| Uniprot Accession Number | Q9P7S5 |
| Absorption | |
| Active Site | ACT_SITE 217; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 812; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (1); Metal binding (12); Modified residue (5); Region (2); Zinc finger (1) |
| Keywords | Hydrolase;Metal-binding;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 333; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517; MOD_RES 337; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517; MOD_RES 486; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517; MOD_RES 493; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517; MOD_RES 645; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19547744; 20473289; 21182284; 21247416; 21712547; 23697806; 24763107; 25452419; 25720772; 27298342; 28410370; 29996109; 30726745; 34296454; |
| Motif | |
| Gene Encoded By | |
| Mass | 98,377 |
| Kinetics | |
| Metal Binding | METAL 56; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 58; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 83; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 86; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 101; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 104; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 109; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 116; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 120; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 127; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 140; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 143; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |