| IED ID | IndEnz0002013237 |
| Enzyme Type ID | protease013237 |
| Protein Name |
Vacuolar-processing enzyme delta-isozyme Delta-VPE EC 3.4.22.34 Asparaginyl endopeptidase delta-VPE |
| Gene Name | dVPE At3g20210 MAL21.23 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSSPLGHFQILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEKMLKDYLSSYIGRNPENDNFTFTESFSSPISNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQACSM |
| Enzyme Length | 466 |
| Uniprot Accession Number | Q9LJX8 |
| Absorption | |
| Active Site | ACT_SITE 164; /evidence=ECO:0000255; ACT_SITE 206; /evidence=ECO:0000255 |
| Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by biotin-YVAD-fmk (a caspase-1 inhibitor) and by Ac-DEVD-fmk. {ECO:0000269|PubMed:15705955}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.; EC=3.4.22.34; Evidence={ECO:0000250|UniProtKB:Q84LM2}; |
| DNA Binding | |
| EC Number | 3.4.22.34 |
| Enzyme Function | FUNCTION: Asparagine-specific endopeptidase that may be involved in processing of proteins targeted to vacuoles (By similarity). Probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:12417707, PubMed:14688293). Exhibits a caspase-1-like activity in extracellular granules. At the early stage of seed development, required for the formation of the seed coat, by regulating cell death of specific cell layers in inner integument (PubMed:15705955). {ECO:0000250|UniProtKB:P49043, ECO:0000269|PubMed:12417707, ECO:0000269|PubMed:14688293, ECO:0000269|PubMed:15705955}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (2); Sequence conflict (1); Signal peptide (1); Site (1) |
| Keywords | Cell wall;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Signal;Thiol protease;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000269|PubMed:15705955}. Secreted, cell wall {ECO:0000269|PubMed:15705955}. Vacuole {ECO:0000250|UniProtKB:Q39119}. Note=Localized to electron-dense structures inside and outside the walls of seed coat cells that undergo cell death. {ECO:0000269|PubMed:15705955}. |
| Modified Residue | |
| Post Translational Modification | PTM: Auto-catalytic activation. {ECO:0000250|UniProtKB:Q84LM2}. |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18849494; 20118269; 22822203; 23858430; |
| Motif | |
| Gene Encoded By | |
| Mass | 52,085 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |