| IED ID | IndEnz0002013248 |
| Enzyme Type ID | protease013248 |
| Protein Name |
Cell wall-associated protease EC 3.4.21.- Cleaved into: Cell wall-associated polypeptide CWBP23 CWBP23 ; Cell wall-associated polypeptide CWBP52 CWBP52 |
| Gene Name | wprA yisM BSU10770 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MKRRKFSSVVAAVLIFALIFSLFSPGTKAAAAGAIDQAAALENGKEQTGAMKEPEQVKWYKVTPGATDIQKNSHMALTVKSDSVLNVSVYPSKEKALKDETFEMYRSFTAEDGKSEVIFPYAWSGPYYVKVEYLGEEEPEDGGTAEAAAEAKYTIGYKGTKKQPSDLEEEEACPVEMSVDQKKSGKGILDKLRSIRDEQLSQTAEGKELTSLYYKAAPFIVAKLALNKTARNEIYQDLVTLKPLFDDVSENGASSSYKVTEKDQKAINRLYDKALQSVPSFLKEEIKKQADRLNMKQLQGKTAGAILTENNIAAKSEVQTTKVIFKVKDNKSLSSVHNEMKGFSASAQSKKDISNVKKAKKLFDNLYSFELPKDEKQNGAYTASAKRVKSAAATLSKMSNVEFAEPVQEYKSLANDIQYPYQWPLKNNGENGGVKNADVKYEPANTLLSKRKLNDTLIAVVDTGVDSTLADLKGKVRTDLGHNFVGRNNNAMDDQGHGTHVAGIIAAQSDNGYSMTGLNAKAKIIPVKVLDSAGSGDTEQIALGIKYAADKGAKVINLSLGGGYSRVLEFALKYAADKNVLIAAASGNDGENALSYPASSKYVMSVGATNRMDMTADFSNYGKGLDISAPGSDIPSLVPNGNVTYMSGTSMATPYAAAAAGLLFAQNPKLKRTEVEDMLKKTADDISFESVDGGEEELYDDYGDPIEIPKTPGVDWHSGYGRLNVMKAVSAADLQLKVNKLESTQTAVRGSAKEGTLIEVMNGKKKLGSAKAGKDNAFKVNIATQKQDQVLYLKATKGDAKTSYKVVVVKGKPSGTPKVNAVKTKDTAVKGKANSKAMIRVKNKSKKVIASAKADAKGTFSVKIKKQKAGTVLYVTAVDTDKKESKEAKVVVEK |
| Enzyme Length | 894 |
| Uniprot Accession Number | P54423 |
| Absorption | |
| Active Site | ACT_SITE 462; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 497; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 650; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by PMSF. {ECO:0000269|PubMed:9004506}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: CWBP52 is a serine-type protease that could be involved in proteoglycan peptide bridges. {ECO:0000269|PubMed:9004506}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (3); Domain (1); Propeptide (1); Sequence conflict (3); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | INDUCTION: In stationary phase. {ECO:0000269|PubMed:11987133}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:10658653, ECO:0000269|PubMed:11987133}. Note=Released into the medium. |
| Modified Residue | |
| Post Translational Modification | PTM: Proteolytically cleaved to yield CWBP23 and CWBP52. |
| Signal Peptide | SIGNAL 1..31; /evidence="ECO:0000269|PubMed:10658653, ECO:0000269|PubMed:9004506" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 21630458; 23180473; |
| Motif | |
| Gene Encoded By | |
| Mass | 96,488 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |