| IED ID | IndEnz0002013271 |
| Enzyme Type ID | protease013271 |
| Protein Name |
Aspartic proteinase yapsin-3 EC 3.4.23.- |
| Gene Name | YPS3 YLR121C L2964 L9233.10 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MKLQLAAVATLAVLTSPAFGRVLPDGKYVKIPFTKKKNGDNGELSKRSNGHEKFVLANEQSFYSVELAIGTPSQNLTVLLDTGSADLWVPGKGNPYCGSVMDCDQYGVFDKTKSSTFKANKSSPFYAAYGDGTYAEGAFGQDKLKYNELDLSGLSFAVANESNSTFGVLGIGLSTLEVTYSGKVAIMDKRSYEYDNFPLFLKHSGAIDATAYSLFLNDESQSSGSILFGAVDHSKYEGQLYTIPLVNLYKSQGYQHPVAFDVTLQGLGLQTDKRNITLTTTKLPALLDSGTTLTYLPSQAVALLAKSLNASYSKTLGYYEYTCPSSDNKTSVAFDFGGFRINAPLSDFTMQTSVGTCVLAIIPQAGNATAILGDSFLRNAYVVYDLDNYEISLAQAKYGTGKENVEVIKSTVPSAIRAPSYNNTWSNYASATSGGNIFTTVRTFNGTSTATTTRSTTTKKTNSTTTAKSTHKSKRALQRAATNSASSIRSTLGLLLVPSLLILSVFFS |
| Enzyme Length | 508 |
| Uniprot Accession Number | Q12303 |
| Absorption | |
| Active Site | ACT_SITE 81; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 288; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Cleaves proteins C-terminally to mono- and paired-basic residues. Required for cell wall integrity. {ECO:0000269|PubMed:10191273, ECO:0000269|PubMed:16087741}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Glycosylation (11); Lipidation (1); Propeptide (2); Region (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Cell membrane;Cleavage on pair of basic residues;Direct protein sequencing;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Signal;Zymogen |
| Interact With | |
| Induction | INDUCTION: Positively regulated in response to cell wall perturbation. {ECO:0000269|PubMed:11016834}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10191273, ECO:0000269|PubMed:11016834}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:10191273, ECO:0000269|PubMed:11016834}. Note=GPI-anchored plasma membrane protein (GPI-PMP). |
| Modified Residue | |
| Post Translational Modification | PTM: Can also be processed to start at Phe-54. {ECO:0000269|PubMed:10191273}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10688190; 11283351; 14561723; 17042746; 19108609; 19536198; 20599573; 22162039; 23029052; 23135325; 27965112; |
| Motif | |
| Gene Encoded By | |
| Mass | 54,570 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |