| IED ID | IndEnz0002013319 |
| Enzyme Type ID | protease013319 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 14 EC 3.4.19.12 Deubiquitinating enzyme 14 UBA domain-containing protein 2 Ubiquitin thioesterase 14 Ubiquitin-specific-processing protease 14 |
| Gene Name | ubp14 ucp2 SPBC6B1.06c |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MSCPHLTETNVVIPDNSQVIYREECVRCFNSQDEEGGIDLCLTCFQSGCGETGLKHSLVHFEQTLHPIVVTIARQPKQKINDEPPQKITKLEIREDSDEDLYDYFYVPKCLVCNIILDIQDPLLSLSLEAMKNATKASNKSQLTAWENELTTCDHIINLPENETYVTNLDNATCSKCDLAENLWMCLTCGALSCGRKQYGGGGGNGHALSHYDDTGHPLAVKLKSISPDGQADIYCYSCDEERIDPNIKTHMLNFGIDIAKLNKTEKSLAELQLEQNLNWDFGASEEDDASKRLFGPGLTGLKNLGNSCYLASTMQSLFSIKEFAIHELNLFNTYNSVCQTPTTDLQCQLGKLADGLVSGKFSKPSKIGLLNNPSSSILPYQDGLRPFMFKDVVGQGHSEFGTSQQQDAYEFLLYLLGKIRKSSIAKTDITKIFDFETEQKLSCLSCKRVRYSSFSSQGLTLTVPRVKIGEIEGEQIYEEVSIDQCLDATIQPDQMEYTCEACKSKLGATTTTAMKSFPKVLILQANRFDLQGYQVKKLSIPIIVNEDGIYNFDRLMAKDHPNDEDYLPEKTETIEWNQSAIEQLQAMGFPLVRCQRALLATGNSDTETAMNWLFEHMEDPEIDKPIEVSELLPKADSSVSEENVQSLCEFGFTVAQARKGLLESNNNIERAVDWILNHPDESFEEPPLEGSDSSIKNENMGSWESTNVPVNYNLKAIISHKGSSAHAGHYVAFIRKEIDGKQQWVLFNDEKVLQVASLEEAKTTGYVYLFERLD |
| Enzyme Length | 775 |
| Uniprot Accession Number | Q11119 |
| Absorption | |
| Active Site | ACT_SITE 309; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 730; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (3); Metal binding (20); Modified residue (1); Zinc finger (2) |
| Keywords | Hydrolase;Metal-binding;Phosphoprotein;Protease;Reference proteome;Repeat;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 456; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18684775; 20473289; 21182284; 21247416; 21760946; 23697806; 23861937; 26412298; 26628015; 29996109; 30726745; 32101745; 34250083; |
| Motif | |
| Gene Encoded By | |
| Mass | 86,783 |
| Kinetics | |
| Metal Binding | METAL 25; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 28; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 41; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 44; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 49; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 56; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 60; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 66; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 153; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 155; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 174; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 177; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 186; /note=Zinc 5; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 189; /note=Zinc 5; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 194; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 207; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 211; /note=Zinc 5; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 217; /note=Zinc 5; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 236; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 239; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |