| IED ID | IndEnz0002013354 |
| Enzyme Type ID | protease013354 |
| Protein Name |
Ubiquitin C-terminal hydrolase 15 EC 3.4.19.12 Deubiquitinating enzyme 15 Protein LARGE GRAIN 1 Ubiquitin thioesterase 15 Ubiquitin-specific protease 15 OsUBP15 |
| Gene Name | UBP15 LG1 Os02g0244300 LOC_Os02g14730 P0503B05.35 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MLQPRESDVPVLFVVFIVLPVIAYFLLGRWHDAVSKKARVSVLAQRAAEETFKVETMATPDVILPGPSLRPMPYMRSAPSARPEYHECATCHGPAKTRCSRCKSVRYCSGKCQIIHWRQGHKQTCQQWNGFGTSSSGGLPPTENTEQMPFLSNLNSPLRGSDVHLHDMDFDTMSEPSFVTTDSYNLDTSPFLSDRSNMNKPNQFLHTSENGAAIGSYEKNDYSIDGEVPSSEILSGNKGLNNSSGSGENCGNRDVIYPLNSVVHQPNNYAPEIRKRPKASITVYESDKGVYLTSDMISSGEGPYASAAESLQRSNSSGNVTGKGNMIHKKPPYPSGKVSSSQKSQEKVLTSHQYDGHEKNPHNKNEQRSTKTAVSTNSSLQGCNGISKAGASKVEALKKPSKFLKTSLVGLINDNKRSKVLFPYEDLVKFFQYEVRGISPRGLFNCGNSCYANAVLQCLMCTKPLMIYLLLRLHSKDCCSKNWCLMCELEQYASTLRESGGPVSPSRILSNLRNIGCRLGGGSQEDAHEFLRHLVMSMQGACLDGLGGEKQVEASLQETTLIQQMFGGRLKSKVKCLRCYHESERYENIMDLTLEIHGWVESLQDALTQFTAPEDLDGENMYKCGRCSAYVKARKQLSVHEVPNILTVVLKRFQTGKYGKINKCVTFPDMLDMVPFVTGAGDNPPLYFLYAVVVHVDTENASFSGHYISYVKDMQGTWLRIDDSEVQAVSLNQVMSEGAYMLFYMRSFPRPPKIYIEKGLSSVPTCSKRHSSKSSKGSKQDLNHTESLFASSDQTYGIYDFRPDNGYIQDQHAALRTRNFYHTDDAFADSISTDFSDATSSEWSLFTSSDESSFTTESTRDSFSVVDYGDNAGLDPISSIFGPYYAQDHPPGSFASCTRLSPSNPQTRYFQENTGFVSDSSMPAHLPGNVHRGRYPDRACSSSAEPPASANPRSVYGRYGLSREGFVQTSGFCQM |
| Enzyme Length | 975 |
| Uniprot Accession Number | Q0E2F9 |
| Absorption | |
| Active Site | ACT_SITE 450; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035; ACT_SITE 706; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:30796160}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (Probable). Involved in the regulation of grain size (PubMed:30796160). Acts as positive regulator of grain width and size by influencing cell proliferation (PubMed:30796160). Functions partially antagonistically with GW2 in the regulation of grain width (PubMed:30796160). Possesses deubiquitinating enzyme activity in vitro (PubMed:30796160). {ECO:0000269|PubMed:30796160, ECO:0000305|PubMed:30796160}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Erroneous initiation (1); Metal binding (8); Zinc finger (1) |
| Keywords | Cytoplasm;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:30796160}. Nucleus {ECO:0000269|PubMed:30796160}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 108,072 |
| Kinetics | |
| Metal Binding | METAL 88; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 91; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 99; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 102; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 108; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 112; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 121; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 125; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134 |
| Rhea ID | |
| Cross Reference Brenda |