| IED ID | IndEnz0002013363 |
| Enzyme Type ID | protease013363 |
| Protein Name |
D-alanyl-D-alanine dipeptidase D-Ala-D-Ala dipeptidase EC 3.4.13.22 |
| Gene Name | vanX SCO3596 SC66T3.07 |
| Organism | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces albidoflavus group Streptomyces coelicolor Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
| Enzyme Sequence | MTGDFAFVDELVSGIRWDAKYATWDNFTGKPVDGYLANRIVGTKALCAALGRAQERAEDLGFGLLLWDGYRPQRAVDCFLRWSQQPEDGRTKARHYPNIGRAEMFDRGYVAARSGHSRGATVDLTLYHLTTGELAAMGGGHDLMDPISHHDARDVPRAEAANRRHLRSIMAACGFASYACEWWHYTLKEEPHPDTYFDFPIA |
| Enzyme Length | 202 |
| Uniprot Accession Number | Q9XAK6 |
| Absorption | |
| Active Site | ACT_SITE 181; /note=Proton donor/acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_01924 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=D-alanyl-D-alanine + H2O = 2 D-alanine; Xref=Rhea:RHEA:20661, ChEBI:CHEBI:15377, ChEBI:CHEBI:57416, ChEBI:CHEBI:57822; EC=3.4.13.22; Evidence={ECO:0000255|HAMAP-Rule:MF_01924, ECO:0000269|PubMed:11881895}; |
| DNA Binding | |
| EC Number | 3.4.13.22 |
| Enzyme Function | FUNCTION: Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide. May play a role in immunity or defense against glycopeptide antibiotics (perhaps at a moderate level) in the soil environment. Might confer vancomycin resistance to S.coelicolor. {ECO:0000255|HAMAP-Rule:MF_01924, ECO:0000269|PubMed:11881895}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Site (1) |
| Keywords | Antibiotic resistance;Cell wall biogenesis/degradation;Dipeptidase;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 22,710 |
| Kinetics | |
| Metal Binding | METAL 116; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01924; METAL 123; /note=Zinc; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01924; METAL 184; /note=Zinc; via pros nitrogen; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01924 |
| Rhea ID | RHEA:20661 |
| Cross Reference Brenda | 3.4.13.22; |