| IED ID | IndEnz0002013418 |
| Enzyme Type ID | protease013418 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 28 EC 3.4.19.12 Deubiquitinating enzyme 28 Ubiquitin thioesterase 28 Ubiquitin-specific-processing protease 28 |
| Gene Name | USP28 RCJMB04_27l24 |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MTAELQAASGGPGGLEADCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIFMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARVSLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK |
| Enzyme Length | 1047 |
| Uniprot Accession Number | Q5ZID5 |
| Absorption | |
| Active Site | ACT_SITE 165; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 601; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome. Deubiquitinates ZNF304, hence may prevent ZNF304 degradation by the proteasome, leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs). {ECO:0000250|UniProtKB:Q96RU2}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (4); Domain (2); Region (4) |
| Keywords | DNA damage;DNA repair;Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 118,747 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |