| IED ID | IndEnz0002013448 |
| Enzyme Type ID | protease013448 |
| Protein Name |
Ufm1-specific protease UfSP EC 3.4.22.- Odorant response abnormal protein 8 |
| Gene Name | odr-8 ufsp-2 F38A5.1 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MTNSQTVSLIGPTQMAPQSTPPPPVNELWFIDAQAMFQNYANLRSFSKSNANEINTTIGGFVFGRKARKQVIHVLFAYAEDLTESNRQFLESSLSADIELVGNLNIDGQSQILPGGQFTLQLTSRMLENRSISEFLDMNVMFNNEHVLMEGASCVSRVGYEWSLRAGREQEDVKSAAERLSMASFRFTYLNAEHGLVIREQKPEAAQQKYLDKFSKGAVPYKDVIEFTAMQSLTRDTSNDTEDQKLVPTVKVTKDNKHFTRLVTIGEVVFPAFFGDSSLDLYKRSREAFNRRANNTMMVTVNGIRAGRGVTTTTSATYLPPGWVSLLHLQLPTKWTDNEQRNYRIRLHKLFNLPSSKPVLRLSQALALHSESARLTNKKLIREPHLSITNYQPVGEITTVNGPYNYHHYMQDGIDDSGWGCAYRSFQTIWSWFILNGYTDKPVPSHREIQQALVDIQDKQAKFVGSRQWIGSTEISFVLNELLKLECRFIATNSGAEVVERVRELARHFETSGTPVMIGGNMLAHTILGVDFNDTTGETKFLVLDPHYTGSEDIKTITSKGWCAWKPASFWSKDHFYNMVLPQPPSDAI |
| Enzyme Length | 589 |
| Uniprot Accession Number | Q94218 |
| Absorption | |
| Active Site | ACT_SITE 421; /evidence=ECO:0000305|PubMed:29251776; ACT_SITE 545; /evidence=ECO:0000250|UniProtKB:Q99K23; ACT_SITE 547; /evidence=ECO:0000305|PubMed:29251776 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (PubMed:29251776, PubMed:24603482). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC (PubMed:9590179). Operates in aggregation behavior, and responses to oxygen levels (PubMed:12410303). {ECO:0000269|PubMed:12410303, ECO:0000269|PubMed:24603482, ECO:0000269|PubMed:29251776, ECO:0000269|PubMed:9590179}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Beta strand (26); Chain (1); Compositional bias (1); Helix (21); Mutagenesis (8); Region (1); Turn (9) |
| Keywords | 3D-structure;Alternative splicing;Chemotaxis;Cytoplasm;Endoplasmic reticulum;Hydrolase;Membrane;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:23449979, ECO:0000269|PubMed:24603482}; Peripheral membrane protein {ECO:0000269|PubMed:24603482}. Cytoplasm {ECO:0000269|PubMed:24603482}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:23449979}. Note=Recruited to the endoplasmic reticulum upon interaction with odr-4. {ECO:0000269|PubMed:24603482}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 5EJJ; 5XDA; |
| Mapped Pubmed ID | 10778742; 12529643; 15338614; 17850180; 21177967; 22267497; 22286215; 22560298; 23800452; 24884423; 25487147; 27240952; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 66,581 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |