| IED ID | IndEnz0002013459 |
| Enzyme Type ID | protease013459 |
| Protein Name |
Beta-fibrinogenase mucrofibrase-5 EC 3.4.21.- Snake venom serine protease SVSP Tm-5 Tm-IIG |
| Gene Name | |
| Organism | Protobothrops mucrosquamatus (Taiwan habu) (Trimeresurus mucrosquamatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Protobothrops Protobothrops mucrosquamatus (Taiwan habu) (Trimeresurus mucrosquamatus) |
| Enzyme Sequence | MVLIRVLANLLILQLSYAQKSSELIIGGDECNINEHPFLVLVYYDDYQCGGTLINEEWVLTAAHCNGENMEIYLGMHSKKVPNKDRRRRVPKEKFFCDSSKNYTKWNKDIMLIRLNRPVRKSAHIAPLSLPSSPPSVGSVCRIMGWGTISPTKVTLPDVPRCANINLLDYEVCRAAYAGLPATSRTLCAGILEGGKDSCGGDSGGPLICNGQFQGIVSWGGDPCAQPHEPGLYTNVFDHLDWIKGIIAGNTDATCPP |
| Enzyme Length | 257 |
| Uniprot Accession Number | Q91511 |
| Absorption | |
| Active Site | ACT_SITE 64; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 109; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 203; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Snake venom serine protease with strong beta-fibrinogenolytic activities, angiotensin I (AGT)-degrading activities and strong kallikrein-like activities in vitro, releasing bradykinin from kininogen (KNG1). Intravenous injection mildly lowers blood pressure in experimental rats, which may be explained by the action on angiotensin I and kininogen. Exhibits amidase activity against N-benzoyl-Pro-Phe-Arg-p-nitroanilide in vitro (PubMed:10973823). {ECO:0000269|PubMed:10973823, ECO:0000269|PubMed:11237764, ECO:0000269|PubMed:7811255}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Heat-stable to about 95 degrees Celsius. {ECO:0000269|PubMed:11237764}; |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (6); Domain (1); Glycosylation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Disulfide bond;Fibrinogenolytic toxin;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Hypotensive agent;Protease;Secreted;Serine protease;Signal;Toxin;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000250 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,164 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |