| IED ID | IndEnz0002013518 |
| Enzyme Type ID | protease013518 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 46 EC 3.4.19.12 Deubiquitinating enzyme Ubiquitin thioesterase Ubiquitin-specific-processing protease |
| Gene Name | usp-46 R10E11.3 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MGATGSSQLEKEISTTESVNNANEHYYGLVNFGNTCYCNSVIQALFFCRPFREKVLNYKQTLKKSGASKDNLVTCLADLFHSIASQKRRVGTIAPKRFITKLKKENELFDNYMQQDAHEFFNYLINTISETLIQEKIAEREKASRHGTLKKGNVTVNLAPATAGLPRSDEKGTSERNGGITVEGNEFLNKSDTTTWIHEIFQGILTNETRCLSCETVSSKDEDFLDLSIDVEQNTSISHCLRVFSETETLCGDQKYFCETCSSKQEAQKRMRIKKPPQLLALHLKRFKFVEPLNRHTKLSYRVVFPLELRLFNVSDDAEYGDRMYDLVATVVHCGATPNRGHYITLVKSNSFWLVFDDDIVEKLEVSSMEEFSGMSTDANIQMPPGNQSAPQKNSESAYILFYQARDYAADDPNHNHKGKNSTHSV |
| Enzyme Length | 426 |
| Uniprot Accession Number | P34547 |
| Absorption | |
| Active Site | ACT_SITE 36; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 342; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Regulates the abundance of the glr-1 glutamate receptor in the ventral nerve cord by promoting its deubiquitination and preventing its degradation in the lysosome. Contributes to the regulation of embryonic polarity. {ECO:0000269|PubMed:21273419, ECO:0000269|PubMed:23209443}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Domain (1); Initiator methionine (1); Lipidation (1); Mutagenesis (1); Region (1) |
| Keywords | Alternative splicing;Cytoplasm;Hydrolase;Lipoprotein;Myristate;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Perikaryon {ECO:0000269|PubMed:21273419}. Cytoplasm {ECO:0000269|PubMed:21273419}. Note=In ventral nerve cord interneurons, localized to discrete puncta within the perikaryon. In the ventral nerve cord, localized diffusely throughout the cytoplasm and to punctate structures. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16049479; 17417969; 21085631; 21177967; 21367940; 22560298; 23800452; 25487147; 32587090; 33622778; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,277 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |