| IED ID | IndEnz0002013539 |
| Enzyme Type ID | protease013539 |
| Protein Name |
PI-stichotoxin-Hcr2l PI-SHTX-Hcr2l Kunitz-type serine protease inhibitor RmIn II Fragment |
| Gene Name | |
| Organism | Heteractis crispa (Leathery sea anemone) (Radianthus macrodactylus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Cnidaria Anthozoa (anthozoans) Hexacorallia Actiniaria (sea anemones) Stichodactylidae Heteractis Heteractis crispa (Leathery sea anemone) (Radianthus macrodactylus) |
| Enzyme Sequence | GSTCLEPKVVGPCKA |
| Enzyme Length | 15 |
| Uniprot Accession Number | P0DV02 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: This recombinant serine protease inhibitor inhibits both trypsin (Ki=2.5 nM) and chymotrypsin (Ki=30 nM) (PubMed:17447883). It may also inhibit the TRPV1 receptor, a receptor of the pain pathway (By similarity). In vivo, shows anti-histamine activity, since it dose-dependently weakens the clinical manifestation of the allergic reaction induced by histamine injection. In vivo, does not show toxicity to mice by intraperitoneal injection (PubMed:17447883). {ECO:0000250|UniProtKB:B2G331, ECO:0000269|PubMed:17447883}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (2); Non-terminal residue (1); Site (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Ion channel impairing toxin;Nematocyst;Protease inhibitor;Secreted;Serine protease inhibitor;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Nematocyst {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,489 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |