| IED ID | IndEnz0002013623 |
| Enzyme Type ID | protease013623 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 46 EC 3.4.19.12 Deubiquitinating enzyme 46 Ubiquitin thioesterase 46 Ubiquitin-specific-processing protease 46 |
| Gene Name | usp46 ch73-16a12.1 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKVQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTVADILQEERKQEKQNGRLKNNGTAIATDTEPEQNKIDPTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEYKYYCEMCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSGDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE |
| Enzyme Length | 370 |
| Uniprot Accession Number | A5WWB0 |
| Absorption | |
| Active Site | ACT_SITE 44; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 317; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:P62068}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action (By similarity). Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity (By similarity). {ECO:0000250|UniProtKB:P62068, ECO:0000250|UniProtKB:P62069}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Erroneous gene model prediction (1); Metal binding (4) |
| Keywords | Behavior;Hydrolase;Metal-binding;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,905 |
| Kinetics | |
| Metal Binding | METAL 186; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P62068; METAL 189; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P62068; METAL 233; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P62068; METAL 236; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P62068 |
| Rhea ID | |
| Cross Reference Brenda |