| IED ID | IndEnz0002013625 |
| Enzyme Type ID | protease013625 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 44 EC 3.4.19.12 Deubiquitinating enzyme 44 Ubiquitin thioesterase 44 Ubiquitin-specific-processing protease 44 |
| Gene Name | usp44 zgc:55661 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MDRCKHVGRLRLAQDHSILNPQKWHCVDCNTTESVWACLSCSHVACGRYIEEHALQHFKEQHHPLALEVNELYVYCYLCDDYVLNDNATGDLKLLRSTLSAIKSQCYEVTTRSGRTLRSSSANGDQLSPSTQELQLRDEDRMFTALWHRRRALIGRLFRLWFAQTERGKKRLEEERQQEEEEERKREARERRRQLKRQLKEELESAPPRKSHRIRRQSLKATSVKPARAPAKSTKSVRRRSAPARVSTPTPRTPRERTPQQRPRPQAKAKRPQAPPSKTGDSPVKRRPTVTPGVTGLRNLGNTCYMNSILQVLSHLHVFRECFLRLDLNQALELLASAVSRKLGLSAQRVIQPKGLNQGSGLSGGASRSRNMELIQPKEPSSKHISLCHELHTLFPVMWSGKWALVSPFAMLHSVWQLIPAFRGYAQQDAQEFLCELLDKVQHELERTRTLTPATVPANQRRLIKQVLSVVNTIFHGQLLSQVRCLACDHRSNTIEPFWDLSLEFPERYHSNSKDAAQVPCGLTEMLAKFTETEALEGAIYACDYCNNKRRRFCSKQVVLTEAQKQLMVHKLPHVLRLHLKRFRWSGRNHREKIGVHVQFEQELNMEPYCCKDSGNLPHPQHFLYQLSAVVMHHGKGFGSGHYTAFCYNTEGGFWVHCNDSKLSVCAVEEVCKAQAYILFYTQRNAQDKSKESDL |
| Enzyme Length | 695 |
| Uniprot Accession Number | Q7ZUM8 |
| Absorption | |
| Active Site | ACT_SITE 304; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 642; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinase that plays a key role in the spindle assembly checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of cdc20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Metal binding (12); Region (1); Zinc finger (1) |
| Keywords | Cell cycle;Cell division;Hydrolase;Metal-binding;Mitosis;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20040115; 28450118; |
| Motif | |
| Gene Encoded By | |
| Mass | 79,926 |
| Kinetics | |
| Metal Binding | METAL 4; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 6; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 26; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 29; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 38; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 41; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 46; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 53; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 57; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 63; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 76; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 79; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |