| IED ID |
IndEnz0002013693 |
| Enzyme Type ID |
protease013693 |
| Protein Name |
Vacuolar protein sorting-associated protein 4
|
| Gene Name |
VPS4 CAALFM_C503090WA CaO19.11814 CaO19.4339 |
| Organism |
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Saccharomycotina (true yeasts)
Saccharomycetes
Saccharomycetales
Debaryomycetaceae
Candida/Lodderomyces clade
Candida
Candida albicans (Yeast)
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
|
| Enzyme Sequence |
MSGASDFLSKGIDLVQKAIDADTATRYEEAYKLYYNGLDYLMLAIKYEKNPKSKELVKSKFTEYLTRAEQLKDHLEKQAQNKSTAESSVNGSTKAKKSNGDGNGSGDDNDDADTKKLRGALAGAILSEKPNVKWSDIAGLDAAKEALKEAVILPVKFPQLFVGNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESERLVKQLFTMARENKPSIIFIDEVDALCGPRGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDAAVRRRFERRIYIALPDVEARTRMFEINIGDVPCECTPHDYRTLAEMTDGYSGHDVAVVVRDALMQPIRKIQQATHFKPVIDETDGKEKLTPCSPGDEGAREMNWMDLATDELKEPPLTIKDFIKAIKNNRPTVNEADIAQHVKFTEDFGQEGN |
| Enzyme Length |
439 |
| Uniprot Accession Number |
Q5AG40 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
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| Calcium Binding |
|
| catalytic Activity |
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| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Pre-vacuolar protein sorting protein involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Required for multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex. Required for extracellular secretion of the secreted aspartyl proteases SAP2, SAP4, SAP5, and SAP6. Its regulation of the pre-vacuolar secretory pathway is critical for virulence. {ECO:0000269|PubMed:15590834, ECO:0000269|PubMed:17506830, ECO:0000269|PubMed:18814053, ECO:0000269|PubMed:19819358, ECO:0000269|PubMed:20026677, ECO:0000269|PubMed:20581294, ECO:0000269|PubMed:22427429, ECO:0000269|PubMed:25483774}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
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| nucleotide Binding |
NP_BIND 175..182; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00136 |
| Features |
Chain (1); Compositional bias (1); Domain (1); Natural variant (2); Nucleotide binding (1); Region (1) |
| Keywords |
ATP-binding;Endosome;Membrane;Nucleotide-binding;Reference proteome;Virulence |
| Interact With |
|
| Induction |
INDUCTION: Expression is regulated by GCN2 and GCN4. {ECO:0000269|PubMed:16215176}. |
| Subcellular Location |
SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:P52917}; Peripheral membrane protein {ECO:0000305}. |
| Modified Residue |
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| Post Translational Modification |
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| Signal Peptide |
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| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
48,437 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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