| IED ID | IndEnz0002013733 |
| Enzyme Type ID | protease013733 |
| Protein Name |
Metacaspase-8 AtMC8 EC 3.4.22.- Metacaspase 2e AtMCP2e |
| Gene Name | AMC8 MCP2E At1g16420 F3O9.22 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAKKALLIGINYPGTAVELRGCVNDVHRMQKCLIELYGFANKDIVIMIDTDKSCIQPTGKNICDELDNLIASGQSGDFLVFHYSGHGTRIPPGIEDSEDPTGFDECITPCDMNLIKDQQFREMVSRVKEGCQLTIISDSCHSGGLIQEVKEQIGESHMKPVDKVKEQIEESHMKQPKLGIASYFLNIVMNLLATCGVSKSQRDRGGGEESFRGEIELEKDETLDIKTRYLPFESYLSLLKEQTGQTNIEPVRIRQTLLKLFGEDPSPNRQRGLSDLGNCEVDAGDSGASRLNAVTDNGILLSGCQTDQRSEDVYVTRTGKAYGAFSDAIQMILSAPRKDKKKITNKELVSEARVFLKKRGYSQRPGLYCHDRFVDKPFICY |
| Enzyme Length | 381 |
| Uniprot Accession Number | Q9SA41 |
| Absorption | |
| Active Site | ACT_SITE 86; /evidence=ECO:0000250; ACT_SITE 140 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease that cleaves specifically after arginine residues. Does not cleave caspase-specific substrates. May be involved in the modulation of programmed cell death activated by oxidative stress. {ECO:0000269|PubMed:17998208}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5-8.5 with t-butoxycarbonyl-GRR-aminomethylcoumarin as substrate. {ECO:0000269|PubMed:17998208}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Modified residue (1); Mutagenesis (1); Sequence conflict (1) |
| Keywords | Autocatalytic cleavage;Hydrolase;Protease;Reference proteome;S-nitrosylation;Thiol protease |
| Interact With | |
| Induction | INDUCTION: By UV-C light, hydrogen peroxide and methyl viologen. {ECO:0000269|PubMed:17998208}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 140; /note=S-nitrosocysteine; /evidence=ECO:0000250|UniProtKB:Q9FYE1 |
| Post Translational Modification | PTM: Proteolytically processed; by an autocatalytic mechanism. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15047898; 15634198; 18467340; 18650403; 20736450; 22112449; 23344059; 27016889; |
| Motif | |
| Gene Encoded By | |
| Mass | 42,521 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |